粗毛淫羊藿不同部位、不同生境中淫羊藿苷、总黄酮含量的变化分析

Variation of icariin and total flavonoid of Epimedium acuminatum in different parts and habitats

目的:考察淫羊藿苷和总黄酮类成分在药用植物粗毛淫羊藿不同部位的代谢分布趋势及其与不同生境的相关性,为其栽培管理与药材采收提供实践指导。方法:对4种生境24个样方中的成年植株采样,分为叶、茎、根、根茎4个部位,用HPLC,UV分别测定各样品的淫羊藿苷、总黄酮含量,SPSS 17.0作数据统计分析。结果:淫羊藿苷在叶、根的含量远高于在茎(地上茎、地下茎)中的含量,黄酮类成分在叶含量高于其他器官,其他器官含量则无差别;不同生境对淫羊藿苷在植株不同部位的积累有一定影响,而黄酮类在不同生境下植株不同部位中的含量差异不明显。结论:淫羊藿苷在植株中的含量分布具有较明显的部位、生境选择性;黄酮类在植株各部位中的代谢与积累则表现较均匀,且受环境影响不大。从淫羊藿苷、黄酮类成分在植株不同部位的分布和积累比例、不同生境下的生长策略综合考虑,采挖粗毛淫羊藿地上部分,既可保证药材的质量合格,也利于资源的合理和可持续利用。

Objective ： Through comparative study on contents of icariin and total flavonoid of Epimedium acuminatum in differ- ent habitats and parts, the distribution and correlation of the two components were observed. Method ： Twenty-four sample spots in four habitats were set up, and the whole plant of these samples was divided as following： leaf, root, stein and rhizome. Total flavonoid and icariin of samples were determined by using the UV method and the HPLC, respectively. The data was analyzed by SPSS 17.0. Re- sult ： The distribution of icariin in different parts had the pattern ： leaf 〉 root 〉 stem 〉 rhizome, the total flavonoid content was high- er in leaf, but it showed no difference in other parts. Habitat had certain effect on icariin accumulation in E. acuminatum. The signifi- cant differences of total flavonoid content in E. acuminatum from different parts were not observed. Conclusion ： The distribution of icariin from different parts and habitats has high selectivity. Metabolism and accumulation of flavonoid content in each part have no difference. Flavonoid content is less affected by environment. Considering the distribution of icariin and flavonoid content in every part and the growth strategy in different habitats comprehensively, it is reasonable to harvest the aerial part for the sustainable utilization of E. acuminatum.