大川芎方效应组分血浆及脑脊液HPLC特征指纹图谱研究

Study on HPLC characteristic fingerprint of active components of Dachuanxiong Fang in plasma and cerebrospinal fluid

目的:通过大川芎方效应组分血浆及脑脊液HPLC指纹图谱的研究,建立准确、简便的体内定性分析方法,为大川芎方入血、入脑成分的研究奠定基础,对阐明其"引药上行"配伍规律提供依据。方法:建立大川芎方效应组分血浆及脑脊液HPLC指纹图谱。结果:各共有峰的相对保留时间的RSD<2.0%,6批血浆、脑脊液样品的指纹图谱的相似度均在0.85以上。结论:所建立的大川芎方效应组分血浆、脑脊液指纹图谱相对稳定、准确,可用于体内成分的定性分析。

Objective： To establish an accurate and simple qualitative analytical method in vivo in the study on HPLC charac- teristic fingerprint of active components of Dachuanxiong Fang in plasma and cerebrospinal fluid, in order to lay a foundation for studies on components found in plasma and cerebrospinal fluid and provide basis for compatible regularity of initiators. Method ： HPLC charac- teristic fingerprint of active components of Dachuanxiong Fang in plasma and cerebrospinal fluid was established. Result ： The similari- ty of fingerprints of all of six batches of samples was above 0. 85, RSD of their relative retention times in common peaks was less than 2. 0%. Conclusion ： The fingerprints of active components of Dachuanxiong Fang in plasma and cerebrospinal fluid were stable and ac- curate that they can be used in qualitative analysis on in vivo components.