贵州省彝族、瑶族及汉族乙型肝炎病毒感染与白细胞介素-10基因微卫星序列的相关研究

Genetic association between interleukin-lO promoter microsatellite polymorphisms and hepatitis B virus infection in Yi, Yao and Han ethnic populations of Guizhou province

目的研究贵州彝族、瑶族及汉族人群白细胞介素-10（IL-10）基因多态性与乙型肝炎（乙肝）病毒（r／By）易感性的相关性。方法采用微卫星DNA聚合酶链反应分析IL-10基因启动子区IL-10．G和IL-10．R两个STR位点在少数民族及汉族人群中的分布，并分析其与HBV感染的相关性。结果IL-10．G与IL-10-R位点的等位基因频率在各民族人群HBV感染组与非感染组之间分布符合H—w遗传平衡定律（P〉0．05），威宁彝族、黔西彝族、荔波瑶族及荔波汉族人群总体HBV感染率为67．00％，各民族感染率分别为51．85％、42．86％、79，52％及84．30％。IL-10．G与IL-10．R两个STR位点多态性在各民族间分布差异有统计学意义（P〈0．05）。IL-10．R多态性在各民族HBV感染组与非感染组之间以及HBV自然清除组与非感染组之间分布差异无统计学意义（P〉0．05）；黔西彝族人群H／3V感染组IL—10．G459bp（19CA）等位基因频率与非感染组相比显著降低（P〈0．05）；荔波汉族人群HBV自然清除组IL-10．G471bp（25CA）等位基因频率与非感染组相比显著降低（P〈0．05）；IL-10．G各等位基因频率在所有民族HBV感染组与自然清除组中分布差异均无统计学意义（P〉0．05），在威宁彝族、荔波瑶族人群HBV感染组与非感染组之间及HBV自然清除组与非感染组之间分布差异也无统计学意义（P〉0．05）。结论IL一10．G、IL一10．R位点多态性在贵州少数民族（彝族、瑶族）以及汉族中有不同的分布，IL一10．G可能与人群对HBV的易感性相关。

Objeetive ： To investigate the association between interleuldn-10 （IL-10） gene promoter microsatellite polymorphisms and the susceptibility to hepatitis B virus infection in Han, Yi and Yao ethnicities in GuiZhou province. Methods 500 volunteers were selected from Guizhou province. Allelic frequency of IL-10.G and IL-10.R loci was identified by short tandom repeat polymerase chain reaction. The relativity between allelic frequency and HBV infection was analyzed. Results Genotype data from H-W analysis on all the IL-10 polymorphisms indicated that it was a random distribution. Very high HBV infection rates were found in the native ethnic minorities of Guizhou province. The overall HBV infection rate among the total population was 67.00%, with the HBV infection rates of i nationality in Weining, Yi nationality in Qianxi, Yao nationality in Libo and Han nationality in Liho as 51.85%, 42.86%, 79.52% and 84.30%, respectively. The polymorphisms distribution of IL-10.G and IL-10.R were statistically different among the ethnic groups （P〈0.05）. The polymorphisms distribution of IL-10.R had no significant difference between HBV infection group and non-infection group, as well as among HBV natural removal group and non-infected group in all the ethnic groups. The frequency of IL-10.G 459 bp （19CA） was significantly higher in non-infection group than in the infected group （ P〈 0.05 ）. The frequency of IL- 10.G 471 bp （25 CA） was significantly higher in the non-infection group than in the HBV natural removal group （P~ 0.05）. The polymorphisms distribution of IL-10.G did not show significant difference between the HBV infection group and the HBV natural removal group in all the ethnic groups. We did not find any differences in allelic and genotypic frequencies of IL-10.G between infection group and non-infection group in Yi nationality in Weining, and Yao nationality in Libo （P〉0.05）, as well as HBV natural removal group and non-infected group （P〉0.05）. Conclusion The polymorphisms distribution of IL-10.R and IL-10.G did not show significant difference in Yi, Yao and Han ethnics population living in Guizhou province. IL-10.G seemed to influence the susceptibility of HBV infection in Han, Yao and Yi ethnics population of Guizhou province.