白芥离体快繁体系的建立

Establishment of rapid propagation system for Sinapis alba in vitro

以白芥幼苗为外植体,MS和1/2MS为基本培养基分别加入不同浓度的6-BA、NAA和KT进行白芥离体再生技术体系研究.结果表明:以白芥幼苗为外植体进行培养,其不定芽增殖效果较好.6种不定芽增殖培养基增殖效果存在明显差异,其中最适宜的芽增殖培养基是MS+1mg/L 6-BA+1mg/L KT,侧芽及不定芽生长迅速、且旺盛,2周后不定芽平均增殖到10个;大量元素含量对幼苗的壮苗具有显著效应,其中3MS培养基的壮苗效果最好,培养21d的苗较对照明显变粗壮;组培苗接种在1/2 MS+0.133mg/L NAA的培养基上诱导生根的效果最佳,茎段培养约15d时发现白色根,主根较多、细长,有侧根,每单株可达7条根.

Rootless seedling of S. alba was used as explants in the experiment. The regenerative system of S. alba were studied through synthesized medium, which composed with MS and 1/2 MS basic medium and different kinds, condentrations of 6-BA, KT and NAA. The results showed that the optimal explants were rootless seedling which used to multiplication of adventitious buds. There were significant differences among 6 different medium in multiplicantion of adventitious bud, and the best multiplication medium was MS＋lmg/L 6-BA＋lmg/L KT. There were significant differences among effects of numerous elements on rejuvenation of plumelet, and 3MS was the best. The best induction rooting medium was 1/2MS ＋ 0. 133mg/L NAA,and 7 roots could be obtained.