肌苷酸合成酶系PURH基因对苏禽乌骨鸡胸肌肌苷酸含量的关联分析

Analysis of the Correlation of PURH Genes Involved in De Novo Purine Biosynthesis on IMP Content in Suqin Silky

在分析氨基咪唑氨甲酰核苷酸转甲酰基酶/次黄嘌呤核苷酸环水解酶基因(fused IMP cyclohydrolasegene/phosohoribosylaminoimidazolecarboxamide formyltransferase PURH)对苏禽乌骨鸡鸡群体肌肉肌苷酸含量的遗传效应。采用PCR-SSCP方法检测苏禽乌骨鸡PURH基因外显子9和外显子16多态性,分析多态位点不同基因型与90日龄胸肌肌苷酸(IMP)含量的关联,各种基因型与胸肌IMP含量的方差分析结果表明:PURH基因外显子16中多态位点T17446C与IMP含量显著相关,TT型个体胸肌IMP含量较CT型高出0.757 mg/g,TT型个体胸肌IMP含量较CC型高出1.083 mg/g,均达到极显著水平(P<0.01),CT型个体IMP含量也显著地高于CC型(P<0.05)。因此,可以利用该基因型对鸡的肉质风味性状进行标记辅助选择。

This experiment was designed to study the genetic effect of polymorpic loci in PURH on breast muscle inosine monophosphate acid（IMP） content in Suqin Silky.The polymorphism sites in exon 9 and exon 16 of PURH on Suqin Silky were identified by PCR-SSCP.Association analysis between the polymorphic sites and IMP contents in muscles of 90 days were tested by least square means.The results showed that TT genotype birds had significant higher IMP content than TC and CC genotype birds（P0.01）,The genotype of PURH gene exon 16（T17446C） had significant genetic effect on IMP content.The IMP content of the individuals with TT genotype was 0.757 mg/g higher than that of the individuals with CT genotype.The IMP content of the individuals with TT genotype was 1.083 mg/g higher than that of the individuals with CC genotype,TC genotype birds also had a significant higher IMP content than CC genotype birds（P0.05）. Therefore the conclusion could the putatively drawn that the genotype could be used as the molecular genetic marker to select the chicken for meat quality trait.