MyoD、Myf6和Mef2c慢病毒过表达载体构建及诱导猪MSC成肌向分化的研究

Construction of MyoD,Myf6 and Mef2c letivirus vector and induced differentiation of porcine MSC cells into myoblasts

骨髓间充质干细胞(mesenchymal stem cells,MSC)是重要的成体干细胞,具有多向分化的能力,其成肌分化能力在医学上有广泛的应用,可用于多种疾病的治疗。成肌决定因子(MyoD)和成肌调节因子6(Myf6)同属生肌决定因子基因家族,控制着肌细胞的增殖分化,肌细胞增强因子(Mef2c)是一个具有重要功能的转录因子,可以作为心肌分化过程中的特异性标志。本试验通过构建猪MyoD、Myf6和Mef2c慢病毒表达载体,并在体外感染猪间充质干细胞(pMSC),成功在pMSC中过表达MyoD、Myf6和Mef2c三种调节因子,并且诱导MSC表达肌细胞生成素(MyoG)、肌肉特异型肌酸激酶(CKM)等重要的成肌相关因子。本研究为利用慢病毒过表达成肌相关因子诱导MSC细胞向成肌细胞分化奠定基础,为进一步探明MSC向成肌细胞分化的分子机制提供发新思路。

As an important source of adult stem cells,mesenchymal stem cells （MSC） had the ability to differentiate. And there was extensive application in medicine and muscle disease treatment.Both of MyoD and Myf6 were family members of myogenic factors, which controled the proliferation and differentiation of muscle cells.Mef2c was an important transcription factor,which could be used as a specific sign in the myocardial differentiation process.The combination of MyoD and Mef2c could induce a large numbers of muscle-specific target gene expression, thereby inducing the cells differentiated into myoblasts.In this study, MyoD, Myf6 and Mef2c lentiviral expression vector were constructed and successfully over- expressed in pMSC.Important myoblast factors,such as MyoG and CKM were increased in MyoD, Myf6 and Mef2c overexpressed pMSC.The results demonstrated that MyoD, Myf6 and Mef2e play an important role in the transition of MSC cells to myoblasts cells.