茶多酚对UVB照射雄性小鼠生殖系统的保护作用

Protective effects of tea polyphenols against the damage of reproductive system in male mice induced by ultraviolet irradiation

研究了茶多酚对中波紫外线（UVB）辐射致雄性生殖系统损伤的保护作用及其可能的机理.将60只雄性小鼠随机分为6组,分别为Ⅰ空白对照组、Ⅱ辐射对照组、Ⅲ~Ⅵ茶多酚实验组.其中Ⅲ~Ⅵ茶多酚实验组上午分别给予5mg kg1d1、10 mg kg1d1、20 mg kg1d1、40 mg kg1d1的茶多酚灌胃1次,Ⅰ空白对照组与Ⅱ辐射对照组分别给予10 ml kg1d1的生理盐水灌胃.Ⅱ~Ⅵ组实验动物均在下午接受3 h的UVB照射.小鼠处理14d后,处死并分别测各组生殖腺重量,睾丸组织SOD活性和MDA含量,睾丸组织切片观察曲细精管变化.结果：与Ⅰ空白对照组相比,Ⅱ辐射对照组小鼠体重和生殖腺重量明显降低（P〈0.05）,睾丸组织SOD活性明显降低（P〈0.01）,MDA含量明显升高（P〈0.01）,睾丸生精上皮排列疏松,生精细胞脱落至管腔.与辐射对照组相比,随着茶多酚剂量的增加,Ⅲ~Ⅵ茶多酚实验组小鼠体重和生殖腺重量明显增加（P〈0.05）,睾丸组织中SOD活性明显增加（P〈0.05）,MDA含量明显降低（P〈0.05）,睾丸生精细胞排列越来越致密.UVB辐射能够造成雄性小鼠生殖系统的损伤,而茶多酚是一种高效的辐射防护剂,能够清除自由基,对受UVB辐射雄性小鼠的生殖系统各脏器和生精细胞具有保护作用.

The protective effects and possible mechanism of tea polyphenols against the damage of reproductive system induced by ultraviolet irradiation was studied.60 mice were divided into 6 groups randomly,Ⅰcontrol group,Ⅱ ultraviolet control group,Ⅲ ~ Ⅵ tea polyphenols groups.Every morning,the mice in Ⅰ and Ⅱ were given 10 ml kg 1 d 1 physiological saline,while mice in Ⅲ ~ Ⅵ groups were given 5 mg kg 1 d 1,10 mg kg 1 d 1,20 mgkg 1d 1,40 mg kg 1 d 1 tea polyphenols by intragastric administration respectively.In the afternoon,Ⅱ ~ Ⅵ groups received 3 hours UVB radiation.Didymus epididymis and seminal vesicle were weighed after completing the experiment,the changes of body weight,didymus,epididymis and seminal vesicle weight were compared.Morphology changes were detected by pathological histology,SOD and MDA changes were detected by method routine.Results： Compared to the control group,the body weight and germen gonad weight degraded obviously（P 0.05）,SOD activity degraded obviously（P 0.01）,MDA content raised obviously（P 0.01）,spermatogenic cells arranged loosely and deciduous spermatogenic cells were found in the lumen of the seminiferous tubules in Ⅱ ultraviolet control group.Compared to Ⅱgroup,with the increase of tea polyphenols dosage,the body weight and germen gonad weight raised obviously（P 0.05）,SOD activity raised obviously（P 0.05）,MDA content decreased obviously（P 0.05）,spermatogenic cells arranged more and more impact in Ⅲ~Ⅵ groups.UVB radiation could cause damage to reproductive system of male mice,and tea polyphenols was an effective anti-radiation protective agent,it can scavenge the free radicals and protect the reproductive organs and spermatogenic cells of male mile under the UVB radiation.