培养基对海洋酵母脂肪酸组成的影响

The effect of culture mediums on fatty acid composition of marine yeast

在海洋酵母基础培养基中添加了海带提取液、KNO3、CuSO4和ZnSO44种因子 ,采用正交试验考察以上因子对海洋酵母脂肪酸组成的影响。研究结果表明 ,各试验组海洋酵母脂肪酸组成及含量均有较明显差异 ,其中KNO3是影响酵母总脂含量和n - 3系列多不饱和脂肪酸 (PUFA)含量的限制因子 ,海带提取液和ZnSO4并列为制约酵母收获量的因素。 4种因子对海洋酵母n - 3系列PUFA组成的影响不大。同时讨论了对微生物脂代谢和净n - 3系列PUFA产量的影响因子 ,n -

Effects of extracted liquid of \%Laminaria japonica\%,KNO\-3,CuSO\-4 and ZnSO\-4 on fatty acid content of marine yeast were studied by means of orthogonal test. Results showed that fatty acid compositions and levels of the marine yeast varied among the treatments. KNO\-3 was the first limiting factor for n-3 series polyunsaturated fatty acids (PUFA) content and total lipid level of the yeast and ZnSO\-4, and extracted liquid of \%Laminaria japonica\% were the second limiting factor affecting yield and net n-3 series PUFA content in the yeast. There was no significant influence on n-3 series PUFA compositions among the treatment. The synthesis path of n-3 series PUFA and factors influencing lipid metabolism and production of n-3 series PUFA in the yeast were discussed.