活血解毒方对糖尿病大鼠神经中AC-cAMP-PKA途径的影响

Influence of Huoxue Jiedu Formula on AC-cAMP-PKA pathway in sciatic nerve of diabetic rats

目的:观察腺苷酸环化酶(AC)-环磷腺苷(cAMP)-蛋白激酶A(PKA)途径在糖尿病大鼠周围神经中的变化及活血解毒方对其的影响。方法:雄性SD大鼠分为正常组、模型组、甲钴胺组、活血解毒方高低剂量组。链脲佐菌素(streptozocin,STZ)腹腔注射65mg/kg造模,放免法检测坐骨神经内cAMP水平,免疫组化法检测坐骨神经中AC、PKA的表达。结果:与正常组比较,模型组大鼠坐骨神经内AC、cAMP水平降低;与模型组比较,活血解毒方高、低剂量组大鼠坐骨神经内AC表达升高,高剂量组cAMP含量升高。结论:AC-cAMP在糖尿病大鼠周围神经中表达水平下降,活血解毒方可能通过调控AC-cAMP途径防治糖尿病神经病变。

Objective： To observe the change of AC-cAMP-PKA pathway in sciatic nerve of diabetic rats and the effect of Huoxue Jiedu Formula on the pathway.Methods： Male SD rats were divided into the normal group,model group,methylcobalamin group,Huoxue Jiedu Formula group.The diabetic rats were established by streptozocin injected intraperitoneally 65mg/kg.The level of cAMP was assessed by radioimmunoassay.The expression of AC,PKA in sciatic nerve were detected using immunohistochemistry.Results： Expression of AC and cAMP were reduced in model group compared with normal group.The expression of AC and cAMP were elevated in Huoxue Jiedu Formula group.Conclusion： The Huoxue Jiedu Formula prevents diabetic neuropathy by promoting AC-cAMP pathway.