健脾益智胶囊法对AD大鼠血清中AchE及海马区GFAP的影响

Experimental study of Jianpi Yizhi method on the serum blood AchE and expression of GFAP in hippocampal region of the rat with synthetic Alzheimer disease

目的:探讨健脾益智胶囊法治疗阿尔兹海默病(AD)的作用机制。方法:通过β-淀粉样蛋白1-40(Aβ1-40)定位注射的方法复制AD大鼠模型,分为空白对照组、假手术组、模型组、中药组、西药组5组,采用酶联免疫法(ELISA)法检测造模4周后和给药4周后血清中乙酰胆碱酯酶(AchE)含量,免疫组化法检测给药后大鼠海马区胶质纤维酸性蛋白(GFAP)阳性细胞数。结果:给药4周后,与模型组比较,中药组和西药组AchE明显降低(P<0.01),GFAP明显升高(P<0.05,P<0.01)。结论:健脾益智胶囊能够抑制AchE的活性,间接提高大鼠鼠血清中Ach含量,并通过升高大鼠海马区GFAP阳性表达,促进星形胶质细胞(As)的激活,达到治疗AD的目的。

Objective： To explore the mechanism of Jianpi Yizhi Capsule in treatment of Alzheimer＇s disease.Methods：The Alzheimer disease model was established by Aβ1-40 injected into hippocamal in rats,and classified into five Groups： the blank control group,sham-operated group,model group,Chinese herbal medicine group（CHM）,western medicine group（WM）.ELISA method was used to measure the serum blood acetylcholin esterase（AchE） after establishing AD model four weeks and administer drugs four weeks,and GFAP expression of astrocytes in hippocampal region was measured with immunohistochemical staining.Results： Comparison with the model group,AchE in CHM,WM was decreased（P〈0.01）,while the number of GFAP positive cell significantly increased（P〈0.05,P〈0.01） after administer drugs four weeks.Conclusion： Jianpi Yizhi Capsule can suppress the activity of AchE,increase the contents of Ach in rats indirectly,and improve the expression of GFAP in hippocampal region,promote activation of astrocytes in the AD rats.Finally,Jianpi Yizhi Capsule has the therapy effect on the treatment of AD.