吴茱萸不同组分镇痛作用及安全范围研究

Study on Analgestic Effect and Safety Scope of Different Components of Evodia Fructus

目的研究吴茱萸不同组分发挥镇痛作用的治疗指数和安全范围。方法采用经典的急性毒性试验方法,进行吴茱萸不同组分的急性致死量试验;采用经典的小鼠热板法,进行吴茱萸不同组分的镇痛药效实验。采用Bliss法,计算各组分的急性毒性试验的LD50和LD5以及发挥镇痛药效的ED50和ED95,求得不同组分发挥镇痛作用的治疗指数(TI),安全系数(SF)。结果吴茱萸挥发油LD50值为2.6995mL.kg-.1d-1,LD5值为1.9853m.lkg-.1d-1;ED50值为0.4625mL.kg-.1d-1,ED95值为0.9996 mL.kg-.1d-1;计算得TI为5.837,SF为1.986。吴茱萸水提组分无法作出LD50,MTD试验结果按含生药量计算为80.0 g.kg-.1d-1;ED50值为1.3515 g.kg-1.d-1,ED95值为4.3435 g.kg-1.d-1。结论吴茱萸水提组分和挥发油组分均有一定的镇痛作用,并呈现一定的量效关系,且吴茱萸水提组分发挥镇痛作用的安全范围大于挥发油组分。

Objective To research on the therapeutic index（TI） and the safety factor（SF） of different components of Evodia Fructus when playing analgestic effect. Methods To make research on the acute toxicity of different components in accordance with classical acute toxicity test methods; to make research on the analgestic effect of different components in accordance with the classical hot plate method; calculating the LD50, LD5, ED50 and ED95 using Bliss method, to calculate the TI and SF of analgestis effect of different components of Evodia Fructus. Results The LD50 of volatile oil in Evodia Fructus was 2.6995 mL·kg-1·d^-1, and the LDs of volatile oil was 1.9853 mL·kg-1·d^-1; the ED50 of volatile oil in Evoclia Fructus was 0.4625 mL·kg-1·d^-1, and the ED95 of volatile oil was 0.9996 mL·kg-1·d^-1; TI was 5.837and SF was 1.986. The water extracts of Evoda＇a Frucrus are unable to make LD50, MTD results calculated in accordance with crude drug content was 80.0 g·kg-1·d^-1, the ED50 was 1.3515 g·kg-1·d^-1, and the EDvs was 4.3435 g·kg-1·d^-1. Conclusion Both the water extracts and volatile oil had certain analgestic effect and had certain dose-efficacy relationship, and the safety scope of water extracts was bigger than volatile oil.