茶多酚EGCG对人骨髓基质干细胞成骨分化基因表达的影响

Effects of tea polyphenols EGCG on the mRNA expression in the osteogenic differentiation of human bone marrow stromal cells

目的:观察茶多酚EGCG对人骨髓基质干细胞成骨分化基因ALK、COL1A1、RUNX2、BMP-2表达的影响。方法:培养人骨髓基质干细胞,用浓度为10-5M、10-6M茶多酚EGCG以及空白对照组分别作用人骨髓基质干细胞21 d后,采用荧光定量PCR的方法检测成骨相关基因ALK、COL1A1、RUNX2、BMP-2的表达变化。结果:BMP-2基因的表达水平为:10-5M茶多酚组>10-6M茶多酚组>DMSO对照组;ALK、RUNX2、COL1A1基因在茶多酚EGCG组与空白对照组之间表达无明显变化。结论:茶多酚EGCG可以提高人骨髓基质干细胞BMP-2基因的表达水平,有可能是茶多酚促进人骨髓基质干细胞成骨分化的重要基因。

Objective： To study the effects of tea polyphenols EGCG on osteogenic differentiation of human bone marrow stromal cells by testing the mRNA expression level.Methods： HBMSCs were cultured in vitro.HBMSCs were treated with 10-5mol/L、10-6mol/L tea polyphenols and blank group for 21 days.The relative expression of osteogenic gene ALK、COL1A1、RUNX2、BMP-2 were detected by real-time PCR with SYBR Green I.Results： The BMP-2 mRNA expression level：10-5M tea polyphenols group〉10-6M tea polyphenols group〉blank group.There was no significant difference in the expression of osteogenic gene ALK、COL1A1、RUNX2.Conclusion：Tea polyphenols EGCG could increase the BMP-2 mRNA expression.It could be the important gene that tea polyphenols enhances osteogenesis in the hBMSCs.