辽宁省间日疟原虫环子孢子蛋白基因型分析

Genotyping of Plasmodium vivax in Liaoning Province via the circumsporozoite protein

目的探讨辽宁省间日疟原虫环子孢子蛋白(CSP)的基因型与地理分布。方法采集镜检确诊的间日疟患者血样15份,Chelex-100离子交换法提取DNA,进行单管-套式PCR扩增,根据琼脂糖凝胶电泳结果判定环子孢子蛋白(CSP)基因型别。结果 12份本地感染间日疟血液标本中6份鉴定为PV-Ⅰ型温带族虫株(占50.00%),6份鉴定为PV-Ⅰ型温带族和PV-Ⅱ型混合虫株(占50.00%);3例输入性间日疟病例中PV-Ⅰ型热带族和温带族各1例,PV-Ⅰ型温带族和PV-Ⅱ型虫株混合感染1例。结论目前辽宁省间日疟原虫存在2种CSP基因型,即PV-Ⅰ型温带族虫株和温带族、PV-Ⅱ型混合感染虫株,无热带族虫株。

Objective To determinate the genotype and characteristics of the circumsporozoite protein of Plasmodium vivax in Liaoning Province.Fifteen blood specimens were collected from patients with P.vivax for DNA preparation.Methods The improved Chelex-100 ion-exchange method was used to extract DNA from blood specimens,and nested and mutation point-specific PCR technique and agarose gel electrophoresis were used to amplify and analyze the resulting fragments.Results In 12 specimens from indigenous cases of P.vivax in Liaoning,6 strains were from the temperate zone family（50.00%） while 6 strains were from a mixed infection of PV-type 2 and the temperate zone family（50.00%）.There were 3 specimens of P.vivax from imported cases;1 strain was from the temperate zone family,1 was from the tropic zone family,and 1 was from a mixed infection of PV-type 2 and the temperate zone family.Conclusion P.vivax circumsporozoite protein exists in two genotypes in Liaoning Province.The genotypes are a strain in the temperate zone family and a mixed infection of PV-type 2 and the temperate zone family,but the tropical zone family strain and PV-type 2 are not present in Liaoning Province.