前列腺素D_2、羧肽酶A3和血小板活化因子在过敏性休克豚鼠体内含量变化

Changes of Prostaglandin D_2,Carboxypeptidase A3 and Platelet Activating Factor in Guinea Pig in Anaphylactic Shock

目的观察白三烯E4(leukotriene E4,LTE4)、前列腺素D2(prostaglandin D2,PGD2)、羧肽酶A3(car-boxypeptidase A3,CPA3)和血小板活化因子(platelet activating factor,PAF)在过敏性休克死亡豚鼠体内的变化规律。方法建立豚鼠过敏性休克的动物模型。ELISA法检测过敏性休克死亡豚鼠尿液中LTE4、血浆中PGD2和CPA3、脑组织中PAF的含量,与对照组比较筛选出有差别的指标。观察比较PGD2、CPA3和PAF在豚鼠过敏性休克0、12、24 h的变化规律,以及血小板活化因子乙酰水解酶(platelet activating factor acetyl-hydrolase,PAF-AH)对豚鼠脑组织中PAF的影响。结果 LTE4的含量在两组间差异无统计学意义,过敏性休克死亡0 h豚鼠体内PGD2、CPA3、PAF含量较对照组明显升高;PGD2和CPA3含量在死后12 h下降至对照组水平,PAF含量死后12、24h仍明显高于对照组;PAF-AH预处理能明显降低PAF含量。结论尿液中LTE4不适宜作为过敏性休克死亡的诊断指标;血浆中CPA3、PGD2及脑组织中PAF在死后一定时间内可作为过敏性休克死亡的法医学诊断指标;PAF-AH有望在临床上用于过敏性休克的治疗。

Objective To detect the changes of leukotriene E4（LTE4）, prostaglandin Dz（PGD2）, carboxypep- tidase A3 （CPA3） and platelet activating factor（PAF） in guinea pigs died from anaphylactic shock. Methods Guinea pigs were used for establishing anaphylactic shock models. The levels of LTE4, PGD2 and CPA3, and PAF were detected in urine, plasma, and brain tissues with ELISA kit, respectively. The significant biomarkers were selected comparing with control group. The changes of PGD2, CPA3 and PAF in the guinea pigs at time zero, 12 and 24 hours after death were observed and compared respectively. The effect of platelet activating factor acetylhydrolase（PAF-AH） to PAF in guinea pig brain was examined and compared. Results There were no statistically differences of LTE4 levels in urine observed between experi- mental group and control group. The levels of CPA3, PGD2 and PAF in the experimental group were sig- nificantly higher than that in the control group at Oh. The levels of PAF at 12 and 24 hours after ana- phylactic shock were significantly higher than that in the control group. The levels of PAF decreased significantly after pretreatment with PAF-AH. Conclusion LTE4 in urine cannot be selected as a biomarker to determine the anaphylactic shock. PGD2 and CPA3 in plasma, and PAF in brain tissue may be used as biomarkers to determine the anaphylactic shock. PAF-AH may be potentially useful for clinical treatment of anaphylactic shock.