良性增生前列腺组织上皮和间质细胞5α-还原酶活性

5a-REDUCTASE ACTIVITY IN STROMA AND EPITHELIUM OF NORMAL AND HYPERPLASTIC HUMAN PROSTATES

对7例正常前列腺和16例良性增生前列腺(BPH)以机械法分离上皮和间质细胞,以氚标睾酮为底物,以TLC法分离酶催化产物,依二氢睾酮(DHT)生成量测定5α-还原酶活性,结果显示:BPH和正常组织间质和上皮部分的酶活性[(pmol DHT/(mg蛋白质·30min)]分别为:91.4±18.1和28.6±7.4及44.7±8.9和23.9±6.8,表明5α-还原酶主要集中在间质部分,BPH组织间质部分酶活性增高,提示5α-还原酶的分布及活性改变与BPH的发生有密切关系。

To study the influence of 5α-reductase on the pathogenesis of human benign prostatic hyperplasia (BPH), the activity of this enzyme was measured in mechanically separated stroma and epithelium from 7 normal and 16 hyperplastic prostates. Samples were incubated in the presence of tritium labelled testosterone. The yield of DHT was used to estimate the enzyme activity. The results showed that the specific activity of the enzyme (pmol DHT / mg protein/30 rain) was91.4±18,1 and 28.6±7.4 in stroma (S) and epithelium (E) of BPH, 44.7±8.9 and 23.9±6.8 in S and E of normal prostates respectively. It indicated that the enzyme is predominantly localized in the stroma and is elevated ia BPH, the primary abnormality of BPH is in the stroma and the increase of 5α-reductase may have some contribution to the pathogenesis of BPH.