十字花科黑腐病菌基因XC0596的突变体构建及其致病性研究

Construction of XC0596 Gene Mutant in Xanthomonas Campestris Pv.campestris and Research of Its Pathogenicity

十字花科黑腐病菌(Xantyomonas campestris pv.campestris,简称Xcc),能引起十字花科植物产生黑腐病。本研究采用自杀质粒pK18Mob介导的整合突变体办法构建了十字花科黑腐病菌Xcc8004菌株中编码假设蛋白的基因XC0596的极性突变体pK0596。对突变体的致病力检测发现这一研究表明,XC0596与十字花科黑腐病菌生长有关,并在致病中发挥重要作用。

Xanthomonas campestris pv.eampestris （Xcc）is the causal agent of black rot disease of crucifers plant, resulting significant reduction in yield and quality. A polar mutant pK0596 of coding the conserved hypothetical protein gene XC0596 of the Xcc 8004 wild strain was constructed by homologous integration with a suicide plasmid pK18mob.The mutant pK0596 showed significandy reduced in virulence compared to wild-type strain, delayed the ability to induce a hypersensitive response （HR） in the nonhost pepper plant （EWC- 10R）.In addition, the growth of the mutant pK0596 in the minimal medium showed that its became smaller and slowly than the wild-type Xcc 8004. This study suggests that the conserved hypothetical gene XC0596 is required for the growth of Xcc 8004 in the minimal medium and plays an important role in virulence of this pathogen.