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趋化因子受体CXCR7在人肝癌细胞株中的表达及意义 被引量:6

Expression and Significance of Chemokine's Receptor CXCR7 in Human Liver Carcinoma Cells
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摘要 目的探讨趋化因子受体(Chemokine receptor,CXCR7)在不同肝癌细胞株中的相对表达强度及意义。方法采用RT-PCR、Western blot法,检测8株不同转移潜能肝癌细胞中趋化因子受体CXCR7在mRNA及蛋白水平的表达情况。结果 CXCR7在8株肝癌细胞中的表达水平差异存在统计学意义,无论是mRNA水平还是蛋白水平,高转移潜能肝癌细胞株(MHCC97-L、MHCC97-H、HCCLM3)CXCR7的表达明显高于普通转移潜能肝癌细胞株(HepG2、PLC/PRF/5、SMMC-7721、BEL-7402、Hep3B),P<0.01。结论 CXCR7与肝癌的发生、发展及预后有一定的相关性。 Objective To investigate the expression and significance of CXCR7 in human liver carcinoma cells. Methods The expression of CXCR7 protein and micro - RNA in hepatocellular carcinoma cell lines ( MHCC97 - L, MHCC97 - H, I-ICCLM3, HepG2, PLC/PRF/5, SMMC - 7721, BEL - 7402, Hep3B ) were de- tected with the application of Western blot analysis and RT - PCR analysis, respectively. Results The ex- pression levels of CXCR7 varied significantly among eight cell lines. Western blotting and RT - PCR showed that high metastatic tumor( MHCC97 -L, MHCC97 -H, HCCLM3 ) cells expressed higher CXCR7 levels than ordinary metastatic tumor ( HepG2, PLC/PRF/5, SMMC - 7721, BEL - 7402, Hep3B) cells ( P 〈 0.01 ). Conclusion CXCR7 may play a key role in occurrence, development and prognosis of liver cancer.
出处 《宁夏医科大学学报》 2012年第6期588-591,共4页 Journal of Ningxia Medical University
基金 宁夏自然科学基金(NZ10153)
关键词 趋化因子受体 CXCR7 肝细胞肝癌 chemokine receptor CXCR7 HCC
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