毛白杨生长素载体蛋白基因PtAUX1超表达的研究

The Study of Over-expression of the Auxin Influx Carrier Gene PtAUX1 in Populus tomentosa Carr.

以毛白杨和拟南芥为研究对象,研究生长素信号转导因子基因在植物发育过程中的作用。利用构建好的PtAUX1的表达载体,转化拟南芥和毛白杨,获得转基因植株,通过PCR技术检测转化植株,分析植株表型并观察植株离体条件下的形态变化。结果显示PtAUX1影响植株各器官的形态发育,毛白杨长势旺盛、外植体有莲座状幼叶产生,拟南芥出现了匍匐生长、不改变根的向地性、茎上的表皮毛增多、花器官畸形等现象。以上结果表明PtAUX1基因通过生长素极性运输,促进各细胞、组织对内源生长素IAA的吸收,进而参与器官极性的建立,影响各器官的形态和发育过程。

The aim was to study the action of genes encoding auxin signaling components in plants development. P. tomentosa and Arabidopsis were chosen as the research object in the paper. PtAUX1 complied with the role of auxin characteristics and influenced organ morphology through the regulation of auxin polar transport in the distribution of the plants. By using expression vector of P. tomentosa PtAUX1 constructed by our laboratory, the auxin influx import carrier PtAUX1 was transformed into Arabidopsis and Poplar. PtAUX1 transgenic plants were detected by PCR, and the phenotypes of transgenic plants and morphologic characters cultured in vivo were analyzed. The experiments showed that the over-expression of PtAUX1 influenced many organs of the morphological development. Transgenic plant of Poplar had vigorous growth and spire. Transgenic plant of Arabidopsis had grown in groveling, not altered root geotropism, trichome number increased on the stem, and abnormal floral organs. The results indicated that PtAUX1 promoted cells and tissues to absorb endogenesis IAA by auxin polar transport, and then participated in organ polarity establishment, and influenced morphology and development of the organs.