快中子诱变小豆‘京农6号’突变体筛选

The Screening of Mutants from 'Jingnong 6' Induced by Fast Neutron in Azuki Bean(Vigna angularisi)

为了进行小豆种质创新和获得基因定位和克隆及其基因功能分析的突变体材料,分别用30、35、40、45、50、55Gy剂量的快中子,辐照小豆‘京农6号’干种子。M1出苗率分别为31.72%、29.06%、30.29%、36.51%、16.15%和15.17%,变异率为3.20%、4.70%、6.75%、6.61%、4.00%和2.20%。辐射剂量增加,出苗率降低、变异率呈增加趋势,M2代共获得77份叶形、株型、粒型、籽粒大小、粒色、叶色、早熟、丰产等性状变异材料。40～45Gy为小豆快中子诱变的适宜剂量,这些突变体为小豆相关基因定位克隆、功能分析和育种提供了有价值的材料。

The aim was to obtain the mutant materials of azuki bean tbr gene location, clone and gene functional analysis. Dry seeds of azuki bean variety ＇Jingnong 6＇ were irradiated with 30, 35, 40, 45, 50, 55 Gy dosage fast neutron respectively for creating new germplasm and obtaining mutants of gene mapping, cloning and functional analysis. Germination rates of M, generation were 31.72%, 29.06%, 30.29%, 36.51%, 16.15% and 15.17% respectively. Their relative variation frequencies were 3.20%, ＇$.70%, 6.75%, 6.61%, 4.00%, 2.20%. The higher dosage of fast neutrons was, the lower germination rate was, and the higher variation frequence was relative. The dosages from 40 Gy to 45 Gy were optimistic to azuki bean fast neutron mutagenesis. Seventy seven mutants including leaf shape, plant architecture, seed shape and size, seed coat color, leaf color, early maturity and high yield in M2 generation were gained. These mutants were very useful for gene mapping, gene cloning, further functional analysis and breeding.