拟巫山淫羊藿种子休眠特性的初步研究

Preliminary Study on Seed Dormancy Characteristics of Epimedium pseudowushanense

为全面掌握拟巫山淫羊藿种子的休眠特性并筛选有效的打破休眠的方法,通过设置不同的处理,观测不同处理条件下胚是否萌动、胚开始萌动所需时间、胚生长和发芽情况。结果表明:拟巫山淫羊藿种子休眠属形态生理休眠(MPD),先暖温(15℃左右)后低温(4℃)、赤霉素处理加低温这2种方法可有效打破其休眠,发芽率皆大于80%;且拟巫山淫羊藿种子休眠可能含形态休眠(MD)和2种类型的生理休眠(PD):暖温(15℃左右)处理有助于解除第1种PD,这样胚才能萌动(胚萌动率高)并生长,单独4℃、27℃处理,胚萌动率极低,分别为20%、6.7%;低温(4℃)能解除MD;而完成形态后熟的种子只有继续低温才能解除第2种PD而萌发,未经过低温的处理组发芽率皆为0%。GA3可以替代最初的暖温破除第一种类型的PD。

To research the mechanism of dormancy and find out the breaking method for the seeds of Epimedium pseudowushanense B.L.Guo,the seeds were treated with different treatments.The time of embryos begin to grow,the growth of embryos and the germination rate with these treatments were observed.The result showed that,the seeds of E.pseudowushanense exhibited morphophysiological dormancy（MPD）,and required warm stratification（15℃） followed by cold stratification（4℃）,or treating with GA 3 followed by cold stratification to break this dormancy,the germination rates were over 80% in these two conditions.As to E.pseudowushanense seeds,two types of PD as well as MD appeared to be involved in the dormancy.Warm temperature was required to break one kind of PD,otherwise embryos could not grow,merely 20%（6.7%） of embryos began to grow in 4℃（27℃） only.The low temperatures were required to break MD,but seeds could not germinate until low temperatures overcome the second type of PD,or the germination rates were 0%.GA 3 could substitute for warm temperatures to break the first kind of PD.