神经导管联合自体变性肌桥修复大鼠周围神经缺损的实验研究

Denatured Autologous Muscle Graft Combining PLGA Nerve Guide Conduits in Repairing Peripheral Nerve Defects

目的探讨PLGA神经导管联合化学萃取的自体骨骼肌肌桥,修复大鼠坐骨神经缺损的可能性。方法SD大鼠45只,建立大鼠左侧坐骨神经缺损模型。随机分为3组,分别采用自体神经(A组)、PLGA神经导管(B组)和PLGA神经导管联合化学萃取自体骨骼肌肌桥(C组),来修复神经缺损。术后通过大体观察、坐骨神经功能指数测定、腓肠肌湿质量恢复率测定、组织学观察和图像分析对比等,检测神经缺损修复情况。结果神经导管联合化学萃取自体骨骼肌肌桥能促进坐骨神经再生,各项指标均优于单纯神经导管移植,但是效果略差于自体神经移植。结论PLGA神经导管联合化学萃取自体骨骼肌肌桥,对大鼠坐骨神经缺损具有良好的桥梁作用和促神经生长的作用。

Objective To explore the feasibility of denatured autologous muscle graft combining PLGA Nerve guide conduits in repairing sciatic nerve defects in rats. Methods Forty-five Sprague-Dawley rats were randomly divided into three groups （n=15） and the left sciatic nerve defects model was established on each rat. Autologous nerve （group A）, PLGA nerve guide conduits （group B） and denatured autologous muscle graft combining PLGA nerve guide conduits （group C） were used to bridge the nerve defects. Microscopic observation, sciatic nerve function index, recovery rate of gastrocnemius wet weight, histological observation and computerized imaging analysis were carried out postoperatively. Results The findings showed that denatured autologous muscle graft combining nerve guide conduits was better than nerve guide conduits, but less effective than autologous nerve transplantation. Conclusion Denatured autologous muscle graft combining nerve guide conduits can promote the peripheral nerve regeneration and repair nerve defects.