血清中miRNA常用提取方法的综合比较

Comparison of current laboratory used of miRNA extraction

目的:对目前实验室常用的4种miRNA提取方法的比较,探讨其临床实用性。方法:使用各自的说明书进行提取miRNA,检测目前血液中公认含量比较高的miRNA--U6,通过RT和Q-PCR后的CT值,进行含量和稳定性比较。结果:miRNeasy Mini Kit-TRIzol的CT值较小,稳定性也较好。结论:各种提取方法有显著差异,但是差异较小,miRNeasy Mini KitTRIzol法相对而言较好。

Objective：Comparison of current laboratory used four kinds of miRNA extraction, to explore its clinical utility. Methods： Respective manual extraction of miRNA to detect the relatively high content of blood recognized the miRNA - U6 RT and Q-PCR CT value, content and stability. Resuhs：CT value of miRNeasy Mini KitTRIzol is the best, mirVana PARIS kit have a good stability. Conclusion：Significant differences in the various extraction methods, but the difference is smaller,and the miRNeasy Mini KitTRIzol relatively better.