小鼠血浆中白藜芦醇含量测定及药动学研究

Content Determination of Quantification of Resveratrol in Mice Plasma and Its Application in Pharmacokinetic Study

目的建立测定小鼠血浆中白藜芦醇的高效液相色谱法,并研究白藜芦醇在小鼠体内的药动学。方法色谱柱为C18柱(150 mm×4.6 mm,5μm),流动相乙腈-水(32∶68),流速为1.0 mL.min-1,检测波长306 nm,血浆样品用乙酸乙酯萃取法预处理。结果白藜芦醇线性范围0.1～10.0μg.mL-1,r=0.999 5。日内RSD<10%,日间RSD<15%,回收率98.0%～102.0%。小鼠灌服给予白藜芦醇主要药动学参数为30 min后血浆药物浓度达峰值,Cmax为3.589μg.mL-1,t1/2α为10.5 min,t1/2β为172.7 min。结论该方法灵敏度高,操作方便,适用于白藜芦醇在小鼠体内的药动学研究。

Objective To develop a HPLC-UV method for quantitative determination of resveratrol in mice plasma and to study the pharmacokineties of resvetrol in mice after oral administration. Methods Chromatographic separation was achieved on an Ultimate AQ- Cls column （150 mm × 4.6 mm, 5 μm） using aeetonitrile-water （32 ： 68, V ： V） as mobile phase at a flow rate of 1.0 mL· min^-1. The UV detection wavelength was set at 306 nm, and the assay was executed at 30 ℃. Results The method was validated and found to be linear in the range of 0. 10 - 10.00μg· mL^-1 The assay accuracy and precision were within the range of 98. 0% - 102. 0%. The main pharmaeokinetic parameters of resveratrol , Cmax was 3. 589 μg · mL^-1 ,tmax was 30 min, tl/2α was 10.5 min, tl/2β was 172.7 min. Conclusion The method was sensitive, simple and successfully applied in the study of pharmacokinetics of resveratrol in mice.