扶正散对免疫功能低下小鼠的保护作用

Protective Effect of Fuzheng Powder on Immunosuppressive Mice Induced by Cyclophosphamide

目的研究扶正散对环磷酰胺所致免疫低下小鼠免疫功能的影响。方法用腹腔注射环磷酰胺(80 mg.kg-1)造成免疫低下小鼠模型,灌胃给予不同剂量的扶正散(2.5,5.0,10.0 g.kg-1)。观察对小鼠免疫器官质量、碳粒廓清指数、溶血素生成、外周血白细胞总数、自然杀伤(NK)细胞活性的影响。结果扶正散中、低剂量组可明显提高免疫低下小鼠的胸腺指数(P<0.05),扶正散中、高剂量组可显著提高免疫低下小鼠的脾指数(P<0.01),扶正散低、中、高剂量组均能显著提高血清溶血素水平(P<0.01),扶正散高剂量组可明显增加小鼠碳粒廓清指数K及吞噬指数α(P<0.05),扶正散中、高剂量组可显著增加外周血白细胞数量(P<0.01),中、高剂量组可显著提高NK细胞活性(P<0.01)。结论扶正散能够增强环磷酰胺所致免疫功能低下小鼠非特异性免疫和特异性免疫功能,对免疫失衡机体具有保护作用。

Objective To investigate the immunomodulatory effect of fuzheng powder on immunodepressive mice induced by cyclophosphamide. Methods The immunosuppressive murine model was induced by intraperitoneally injecting cyclophosphamide at the dose of 80 mg·kg^-1 , then they were treated with different doses of fuzheng powder （ 2.5, 5.0, 10.0 g·kg^-1 ） by intragastric administration, and the mass of immune organs, carbon clearance index, serum hemolysin, the total WBC in peripheral blood and NK cell activity were measured. Results Thymus index （ P 〈 0. 05 ） was remarkably increased in thefuzheng powder at middle- and low-dose groups of immunosuppressive mice, while the spleen index （P〈0.01） , the entire number of WBC in peripheral blood（P〈0.01 ）and NK cell activity（ P〈0. 01 ）in the middle- and high-dose groups, and each dose of fuzheng powder raised the levels of serum hemolysin（ P〈0.01 ）. In addition, Fuzheng powder at high-dose played an important role in raising the carbon clearance index K and phagocytic index α of the immunodeppressive mice （P〈 0.05）. Conclusion Fuzheng powder could enhance the nonspecific and specific immunity of immunosuppressive mice induced by cyclophosphamide and also protect immune system imbalance.