正交实验法优选苦瓜根总皂苷提取工艺

Optimization of Extraction Process for Saponins in the Roots of Momordica charantia.L by Orthogonal Design

目的优选苦瓜根中苦瓜总皂苷的最佳提取工艺。方法以提取液中苦瓜总皂苷含量为考察指标,分光光度法测定其含量,采用L9(34)正交实验法优选苦瓜根总皂苷的提取工艺。结果苦瓜根中苦瓜总皂苷较优的提取工艺为提取2次,每次2 h,乙醇浓度60%,乙醇用量8倍,提取温度80℃。结论优选得到的提取工艺稳定、可行、重复性好,可为苦瓜根总皂苷的提取提供参考。

Objective To optimize the technology of extraction for saponins in the roots of Momordica charantia. L. Methods The total saponins of Momordica charantia. L and Ginsenoside were taken as indexes, which were detected by spectrometric method. And optimum extraction technology for the total saponins was studied by the L9 （ 3^4 ） orthogonal test.Results The optimum extraction technology was obtained as extracting twice, two hours for each, with 8 times 60% alcohol at 80 ℃. Conclusion The optimized technology is stable, feasible and reproducible,which provides evidence for total saponins extraction from the roots of Momordica charantia. L.