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外源基因插入位点旁侧序列的研究方法及进展 被引量:2

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摘要 外源基因插入位点的研究,是转基因植株分子检测的一项重要内容。本文综述了近些年用于旁侧序列测定的染色体步移技术及其发展,介绍了基于基因组文库和PCR的染色体步移技术。同时总结了基因组文库步移、反向PCR、锅饼PCR、T-linker PCR和TAIL-PCR的原理及其特点,以期在实验方法的选择上提供参考意见。
作者 刘蓓
出处 《农业与技术》 2012年第4期97-97,共1页 Agriculture and Technology
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