杜仲皮和叶中儿茶素含量差异的研究

Comparison of Catechin Content in the Barks and Leaves of Eucommia ulmoids Oliv.

[目的]研究杜仲皮和叶中儿茶素含量的差异,为杜仲以叶代皮入药提供依据。[方法]选取3个产地的杜仲皮和叶样品进行提取,并采用反相高效液相色谱法测定儿茶素的含量,色谱柱为Agilent HC-C18(4.6 mm×250 mm,5μm);流动相为乙腈-浓度0.4%磷酸溶液(13∶87,V/V);流速为1.0 ml/min,检测波长280 nm。[结果]所取3个产地的同株杜仲的皮和叶中儿茶素的含量不同,其中河南信阳、四川绵阳和贵州遵义产的杜仲皮中儿茶素的含量分别为1.919、1.279和1.167 mg/g,所产的杜仲叶中儿茶素的含量分别为49.34、35.75和33.72 mg/g;同株杜仲叶中儿茶素含量明显高于杜仲皮。[结论]研究所采用分析方法简便、快速,重现性良好,适用于杜仲不同部位儿茶素含量的测定。测定结果表明杜仲叶资源值得开发利用。

[Objective] To discuss the different content of catechin in the leaves and barks of E.ulmoides,so as to provide references for using its leaves instead of barks as medicine.[Method] The E.ulmoides barks and leaves from three different places were collected and then extracted,their content of catechin was determined by RP-HPLC on a column of Agilent HC-C18（4.6 mm×250 mm,5 μm）,using the mobile phase of acetonitrile-0.4% phosphoric acid solution（13∶ 87） at a flow rate of 1.0 ml/min and with the detection wavelength of 280 nm.[Result] The content of catechin in the barks and leaves of E.ulmoids was different in the three places,the catechin contents in the barks of E.ulmoides produced in Xinyang of Henan,Mianyang of Sichuan and Zunyi of Guizhou were 1.919,1.279 and 1.167 mg/g,while that in the leaves were 49.34,35.75 and 33.72 mg/g;the catechin content in leaves was significantly higher than that in barks.[Conclusion] The analytic method in this study is simple and rapid with good reproducibility,thus is applicable to the determination of catechin content in different parts of E.ulmoides.The determination results proved that the E.ulmoides leaves is worthy to be exploited.