福建金线兰多倍体的诱导和倍性鉴定

Induction and Ploidy Identification of the Polyploid of Anoectochilus fornosanus

[目的]对福建金线兰多倍体进行诱导和倍性鉴定。[方法]以福建金线兰为材料,以秋水仙素为诱变剂,分别用浸泡法和涂抹法处理福建金线兰试管苗不定芽,进行多倍体诱导,并在显微镜下观察鉴定其染色体的倍性。[结果]以浓度0.05%秋水仙素为诱变剂,用涂抹法处理72 h的效果较佳,加倍率为35%;对多倍体材料进行染色体鉴定发现,二倍体染色体为2n=40条,四倍体为4n=80条。[结论]该方法可以培育福建金线兰多倍体,能提高其移栽成活率和亩产量。

[Objective] To induce the polyploid of Anoectochilus fornosanus and identify its ploidy.[Method] The adventitious buds of Anoectochilus fornosanus were respectively soaked and coated with colchicine to induce the polyploid,and the ploidy of the chromosome was observed under microscope.[Result] The ploidy was 35% when the adventitious buds were coated with 0.05% colchicine for 72 h;the ploidy identification showed that there were 40 diploid chromosomes and 80 tetraploid chromosomes.[Conclusion] The method can cultivate the polyploid of Anoectochilus fornosanusand,improve its survival rate and per mu yield of its transplants.