藏药桃儿七的快繁体系及其鬼臼毒素含量分析

Rapid Propagation System of Tibetan Medicine Sinopodophyllum hexandrum and Determination of Its Podophyllotoxin Content

[目的]建立藏药桃儿七的组培-移栽体系,并比较野生桃儿七与组培桃儿七的根茎中鬼臼毒素的含量差异。[方法]以藏药桃儿七种子为外植体诱导愈伤组织,将组培苗炼苗移栽,并利用高效液相色谱技术(HPLC)测定野生品桃儿七与组培品桃儿七的根茎中鬼臼毒素的含量。[结果]桃儿七带胚种段在MS+1.0 mg/L 2,4-D+0.5 mg/L GA3培养基上愈伤诱导率最好,达33.33%;组培苗在蛭石基质中存活较好,成活率可达80%以上;西藏野生品桃儿七根茎中鬼臼毒素含量为1.01%,组培品中含量为0.087%。[结论]通过组织培养可在不破坏野生资源的前提下生产鬼臼毒素,该研究为桃儿七引种栽培提供了试验指导。

[Objective] To establish the tissue culture-transplanting system of Tibetan medicine S.hexandrum,and compare the podophyllotoxin content in rhizomes between wild and cultured S.hexandrum.[Method] The seeds of S.hexandrum were used as explants to induce callus,and the seedlings were acclimatized and transplanted,the podophyllotoxin content in rhizomes of wild and cultured S.hexandrum was determined with HPLC.[Result] The seeds on medium of MS＋1.0 mg/L 2,4-D ＋ 0.5 mg/L GA3 got the best callus induction rate of 33.33%;the vermiculite was the best matrix for seedlings to survive,and the survival rate could reach up to more than 80%;podophyllotoxin content in wild S.hexandrum rhizomes was 1.01%,and that in cultured S.hexandrum rhizomes was 0.087%.[Conclusion] Through tissue culture,the podophyllotoxin can be produced without damaging wild resources,the study provides guidance for the introduction and cultivation of S.hexandrum.