津鲢的同工酶分析

The Isozyme Analysis of Jin Silver Carp(Hypophthalmichthys molitrix Var Jin)

[目的]对津鲢进行同工酶分析。[方法]使用水平式淀粉凝胶电泳法,对选育品种津鲢进行AAT、EST、α-GPD、GPI、IDH、LDH、MDH、ME、PGM和PROT共10种同工酶及蛋白质的电泳分析,并以购自湖北省荆州市的鲢人工繁殖群体进行比较。[结果]以肌肉和肝脏作为检测用组织,共检测出18个基因座位;在津鲢群体中有变异的基因座位为GPI*和PGM*,荆州鲢群体中有变异的基因座位为GPI*;津鲢与荆州鲢的多态基因座位(最高基因频率≤0.99)比例和平均杂合度预期值分别为11.11%、5.56%和0.015 0、0.001 1,群体间Nei遗传距离为0.000 59;2群体GPI*基因座位最高基因频率间的χ2检验结果表明这2个群体间呈极显著差异。[结论]为津鲢的大面积推广奠定理论依据。

[Objective] The aim was to carry out isozyme analysis of Jin silver carp（Hypophthalmichthys molitrix Var Jin）.[Method] The isozyme of AAT,EST,α-GPD,GPI,IDH,LDH,MDH,ME,PGM and PROT of muscles and liver in two populations of the silver carp（Hypophthalmichthys molitrix）： Jin silver carp（a breed through selective breeding） and artificially propagated population bought from Jingzhou City,Hubei province were examined by horizontal starch gel electrophoresis.[Result] 18 loci were observed in two populations.Two loci of GPI＊ and PGM＊ in Jin silver carp population and the locus of GPI＊ in Jingzhou population were polymorphic.The proportions of polymorphic loci（maximum gene frequency ≤ 0.99） of Jin silver carp and Jingzhou populations were 11.11% and 5.56% respectively,expected heterozygosity were 0.015 0 and 0.001 1 respectively.The Nei＇s genetic distances were 0.000 59 between two populations.The result of chi-square test of the GPI＊ gene in two populations showed that their genetic structure has very significant difference.[Conclusion] This study had provide a theoretical basis for large-scale spread of Jin silver carp.