正交设计优选薏苡茎多糖的酶提取工艺

Optimization of Enzyme Extracting Process of Polysaccharides from Coix lachrymal-jobi L. Stems by Orthogonal Design

[目的]确定薏苡茎多糖的最佳酶提取工艺。[方法]利用纤维素酶从薏苡茎中提取多糖,采用正交试验方法进行条件优选,并用分光光度法对提取物进行多糖的含量测定。[结果]检测波长为490 nm;葡萄糖含量在0.010～0.100 mg/ml范围内与吸光度呈良好的线性关系(R=0.999 8);最佳酶提取工艺为:pH值为5,酶用量为6.0 ml,反应温度为40℃,酶解时间为150 min;在此条件下,薏苡茎多糖的平均提取率为19.62%,RSD为0.21%。[结论]优选出的酶提取工艺合理,经济,可行。

[Objective] To determine the optimal enzyme extracting process of polysaccharides from Coix lachrymal-jobi L.stems.[Method] The cellulase extraction process of polysaccharides from C.lachrymal-jobi stems was optimized by means of orthogonal test design.And the content of polysaccharides in extracts was determined by spectrophotometry.[Result] The wavelength of the maximum absorption was 490 nm.Glucose concentration showed a good linear relationship with absorbance at a range of 0.010 to 0.100 mg/ml（R=0.999 8）.The optimal technological conditions for the extraction of polysaccharides were as follows： pH value was 5,amount of enzyme was 6 ml,the reaction temperature was 40 ℃,and enzymatic hydrolysis time was 150 min.Under these conditions,the mean extraction rate of polysaccharides was 19.62% with RSD of 0.21%.[Conclusion] The enzyme extracting procedure is reasonable,economical and feasible.