林蛙皮活性肽对小鼠免疫功能影响的试验研究

Experimental Investigation of Bioactive Peptides from the Skin of Rana chensinensis on Immune Function in Mice

[目的]探讨林蛙皮生物活性肽对正常小鼠免疫功能的影响。[方法]将小鼠随机分为阴性对照组、阳性对照组、林蛙皮活性肽低、中、高剂量组,每组10只,连续灌胃给药28 d后,采用ConA诱导的小鼠脾淋巴细胞转化试验,检测T淋巴细胞增值,并通过溶血空斑试验和血清溶血素试验检测林蛙皮活性肽对小鼠体液免疫功能的影响。[结果]林蛙皮生物活性肽可明显促进ConA诱导的淋巴细胞增值,增加抗体生成细胞数量,增强血清溶血素抗体效价水平。[结论]林蛙蛙皮生物活性肽对小鼠的细胞免疫和体液免疫功能均具有增强作用。

[Objective] To observe the effects of bioactive peptide from the skin of Rana chensinensis on immune function in normal mice.[Method] Mice were randomly divided into five groups,including negative group,positive group,low-dose group,middle-dose group and high-dose group,each group of ten.The mice were administrated for 28 days.Cellular immunity was detected by T-cell-proliferation MTT assay,humoral immunity was assessed by plaque forming cell assay（PFC） and number of antibody-producing cells.[Result] Bioactive peptide from the skin of Rana chensinensis could obviously promote lymphocyte proliferation induced by ConA,increased antibody production in cell number,and enhanced serum hemolysin antibody titer.[Conclusion] Bioactive peptide from the skin of Rana chensinensis could enhance cellular immunity and humoral immunity of mice.