摘要
目的:观察3个月有氧运动对青年男性外周血淋巴细胞线粒体与细胞核DNA修复酶的影响,探讨有氧运动提高免疫功能的机制。方法:34名健康青年男性随机分为运动组(n=16)和对照组(n=18),运动组进行每周3次共12周的有氧运动干预,对照组保持日常生活习惯不变。实验前后分别测定淋巴细胞凋亡率、淋巴细胞线粒体和细胞核8-羟鸟嘌呤(8-oxoguanine,8-oxoG)、8-羟鸟嘌呤DNA糖基化酶α(8-oxoguanine DNAglycosylaseα,OGG1α)和OGG1β蛋白表达量。结果:两组基础值均无显著性差异。与实验前比较,实验后运动组淋巴细胞凋亡率显著性降低(5.10±0.89 vs 3.64±1.07,P<0.01),线粒体与细胞核8-oxoG均显著性降低(线粒体:8.75±1.85 vs 5.16±0.86,P<0.01;细胞核:3.93±1.35 vs 2.13±0.51,P<0.01),OGG1α和OGG1β均显著性升高(OGG1α:1.26±0.39 vs 2.31±0.42,P<0.01;OGG1β:0.83±0.17 vs 1.45±0.48,P<0.01),而对照组各指标均无显著性变化(均为P>0.05)。结论:规律有氧运动上调了淋巴细胞线粒体与细胞核DNA修复酶的表达量,降低了DNA氧化损伤,淋巴细胞凋亡率随之降低。
Objective： To observe the effects of three - month aerobic exercise on mitochondria and nucleus DNA repair enzymes of peripheal blood lymphocyte in young male subjects and investigate the underlying mechanism of enhanced immune function by aerobic exercise.Methods： Thirty -four healthy young male subjects randomly divided into exercise group （ EG,n=16） and control group （CG, n = 18）. EG conducted a 12-week （3 times per week） aerobic exercise protocols and control group maintained their routine lifestyle. Lymphocyte apoptosis rate, mitochondria and nucleus 8-oxoguanine （8-oxoG） and 8-oxoguanine DNA glycosylase α （OGG1α） and OGG1β protein level were determined before and after intervention. Results ： There were no significant difference of base values between two groups. After intervention, lymphocyte apoptosis rate was decreased （5.10 ± O. 89 vs 3. 64 ± 1.07, P 〈0.O1 ）, mitochondria and nucleus 8 - oxoG were both reduced （mitochondria： 8.75 ±1.85 vs 5.16 ：0.86, P〈0.01 ; nucleus： 3.93 ±1.35 vs 2. 13 ±0.51, P 〈0.01）, OGG1α and OGG1α ± were raised （OGG1α： 1.26±0.39 vs 2.31 ±0.42, P〈0. 01; OGG1β：0.83 ±0. 17 vs 1.45 ±0.48, P 〈0.01 ） in EG compared with pre- exercise, whereas all indicators of CG did not change significantly （ all P 〉 0.05 ）. Conclusion ： Regular aerobic exercise reduced lymphocyte DNA damage and lymphocyte apoptosis rate via upregulation of lymphocyte mitochondria and nucleus DNA repair enzymes protein expression.
出处
《山东体育科技》
北大核心
2012年第3期39-43,共5页
Shandong Sports Science & Technology
