摘要
对AOT/异辛烷反胶束体系萃取油莎豆蛋白的前萃工艺进行了研究,考察了加料量、萃取温度、时间、AOT质量浓度、W0、KCl浓度、pH对蛋白质前萃率的影响,通过单因素试验和均匀设计试验,得到前萃的最佳工艺条件为:加料量0.05 g/mL,AOT质量浓度0.12 g/mL,W0为16,KCl浓度0.02 mol/L,pH7,萃取温度40℃,萃取时间86 min.
In this paper, we studied the forward extraction process AOT/isooctane reverse micelle system, and investigated the effects of of protein from Cyperus esculentus by addition amount, extraction temperature, time, AOT mass concentration, W0, KCl concentration, and pH on the forward extraction rate. Single factor tests and uniform design tests showed that the optimum forward extraction conditions were as follows: addition amount 0.05 g/mL, AOT mass concentration 0.12 g/mL, W0 16, KC1 concentration 0.02 mol/L, pH 7, extraction time 86. min and temperature 40 ℃.
出处
《河南工业大学学报(自然科学版)》
CAS
北大核心
2012年第3期36-39,共4页
Journal of Henan University of Technology:Natural Science Edition
基金
国家自然科学基金(20976037)
河南省杰出人才创新基金(05210005000)
关键词
反胶束
蛋白质
油莎豆
均匀设计
reverse micelle
protein
Cyperus esculentus
uniform design