哮喘小鼠肺组织中基质细胞衍生因子1的表达及布地奈德雾化液的干预作用

Expression of Stromal Cell Derived Factor-1 in Lung of Asthmatic Mice and Effects of Budsonide Suspension

目的探讨哮喘小鼠肺组织中基质细胞衍生因子1(SDF-1)的表达及布地奈德雾化液干预的影响。方法清洁级雌性昆明小鼠30只,随机分为对照组、哮喘组和布地奈德雾化液干预组,每组10只。建立卵白蛋白致敏的哮喘小鼠模型并用布地奈德雾化液进行干预,用免疫组织化学方法检测各组小鼠肺组织中SDF-1的表达,分别用HE染色和Wright-Giemsa染色方法对各组小鼠气道及支气管肺泡灌洗液(BALF)中炎症细胞情况进行评估。结果 SDF-1在对照组中呈低表达(0.21±0.02),在哮喘组中表达明显增加(0.48±0.03),使用布地奈德雾化液进行干预后SDF-1表达明显降低(0.29±0.01),差异有统计学意义(P<0.01)。哮喘组气道炎症细胞浸润程度明显高于对照组,干预后降低;哮喘组中BALF中炎细胞总数及嗜酸粒细胞、淋巴细胞、中性粒细胞计数均高于对照组,布地奈德雾化液干预后炎性细胞总数及各分类计数均降低,差异有统计学意义(P<0.05)。结论SDF-1在哮喘小鼠气道炎症细胞募集中可能有重要作用,布地奈德雾化液减轻哮喘小鼠气道炎症可能与降低SDF-1的表达有关。

Objective To investigate the expression of stromal cell derived factor-1 （ SDF-1 ） and the effects of budesonide suspension for inhalation （Pulmicort Respules） in mice with asthma. Methods Thirty Kunming female mice were randomly divided into three groups,ie, a control group, an asthma group, and a pulmicort treatment group. The asthma group and the pulmicort treatment group were sensitized with ovalbumin （OVA） by a combination of intraperitoneal injection and repeated OVA intranasal challenges to establish mouse asthma model. The pulmicort treatment group received 1001xL pulmicort by intranasal administration before OVA challenge. The immunohistochemistry was used to estimate the expression of SDF-1 in lung tissues. HE staining and Wright-Giemsa staining method were used to assess inflammatory infiltration in the airway and bronchoalveolar lavage fluid （BALF） respectively. Results The expression of SDF-1 in the asthma group increased significantly compared with the control group （0. 48±0.03 vs. 0. 21±0. 02,P 〈0.05） ,and significantly decreased after the intervention with pulmicort （0. 29±0. 01 vs.0. 48 ＋ 0. 03, P 〈0. 05 ）. Compared with control group, the infiltration of inflammatory cells in airway was significantly enhanced in the asthma group, and attenuated in the pulmicort treatment group. The total number of inflammatory cells and eosinophil, lymphocyte, neutrophil counts in BALF increased significantly in the asthma group compared with the control group, and decreased significantly after pulmicort intervention. Conclusion SDF-1 may play an important role in the recruitment of inflammatory cells in asthmatic airway and pulmicort may relieve airway inflammation by decreasing the expression of SDF-1.