补脾益气方药对哮喘大鼠嗜酸性粒细胞趋化因子表达的影响

Effect of traditional Chinese medicine prescription for Reinfocing Pi Qi on the expression of eotaxin in asthmatic rat model

目的观察补脾益气方药对哮喘大鼠肺组织嗜酸性粒细胞趋化因子(eotaxin)表达的干预作用。方法SPF级雄性SD大鼠随机分为对照组(A组)、哮喘组(B组)、生理盐水治疗组(C组)、地塞米松组(D组)和补脾益气方药治疗组(E组)。哮喘组通过卵蛋白(OVA)致敏、激发建立大鼠哮喘模型,以生理盐水和地塞米松分别做对照治疗。E组大鼠在复制哮喘模型后,按照1 mL/100 g体质量给予补脾方药1次/d,共14 d。计数肺泡灌洗液细胞总数、嗜酸性粒细胞(EOS)、巨噬细胞、淋巴细胞和中性粒细胞数;光镜下观察肺组织病理学改变;采用RT-PCR和Western bloting检测肺组织eotaxin的表达。结果与A组比较,B、C组肺泡灌洗液EOS计数、eotaxin的mRNA和蛋白表达均增加(P<0.01),D、E组较B组降低(P<0.01),D、E组间无差异(P>0.05)。结论补脾益气方药可能通过降低哮喘大鼠体内eotaxin水平,减轻炎症细胞在肺组织局部浸润,抑制气道炎症。

Objective To observe the intervention influence of traditional Chinese medicine （TCM） prescription for Reinfocing Pi Qi on the expression of eotaxin in asthmatic rat, and to explore its possible mechanism. Methods SPF male SD rats were randomly divided into normal control group （A group）, asthmatic group （B group）, normal saline group （C group）, therapeutic group of Dexamethasone （ D group）, therapaeutic group of TCM prescription for Reinforcing Pi Oi （ E group）. The asthmatic models were established by the classic way of OVA sensitization and excitation. Dexamethasone and normal saline were used as positive and negative control medicine. TCM prescription for Reinforcing Pi Qi were taken once a day in 1 mL/100 g for 14 days after seting up model in the rats of E group. The number of total ceils, percentages of eo- sinophil （EOS）, lymphocyte, neutrophil in bronchoaleolar lavage （BALF） fluid were calculated. Pathological change of lung tissue were observed. The expression of eotaxin on level of mRNA and protein in lung tissue were detected by RT-PCR and Western blot assay. Results EOS number of BALF and expression of eotaxin in B group and C group were higher than those in A group （P 〈 0.01 ）. EOS number of BALF and expression of eotaxin in D group and E group were lower than those in B group （P 〈0.01 ）. However, there was no difference between D group and E group （P 〉0.05）. Conclusion TCM prescription for Reinforcing Pi Qi can decrease the level of eotaxin in asthmatic rats, and alleviate inflammatory cells infiltration in lung, then inhibit airway inflamation.