MMP-9和NF-κB在视网膜母细胞瘤中的表达

Expression of MMP-9 and NF-κB in retinoblastoma

目的:研究基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)和核转录因子-κB(nuclear factor of κB,NF-κB)在视网膜母细胞瘤(Retinoblastoma,RB)中的表达及与RB分化程度和视神经浸润的关系,探讨它们在RB浸润、转移过程中的作用。方法:应用MMP-9和NF-κB单克隆抗体,采用免疫组织化学方法,对47例RB患者石蜡包埋标本进行MMP-9和NF-κB表达的测定,数据进行统计学分析。结果:(1)47例RB中MMP-9阳性表达率为46.8%,与RB的分化程度(P<0.05)及有无视神经浸润(P<0.05)相关。(2)NF-κB在RB组织中阳性表达率为63.8%,与RB的分化程度相关(P<0.05),与有无视神经浸润无相关性(P>0.05)。(3)RB中NF-κB的表达与MMP-9的表达有相关性(P<0.05)。结论:RB组织中MMP-9高表达。NF-κB在RB中被激活。RB中NF-κB表达与MMP-9表达相关,NF-κB激活后可能通过上调MMP-9的表达促进RB的浸润、转移。二者表达的检测可以作为反映RB浸润、转移潜能的生物学指标,有助于筛选转移高危病例。

AIM： To investigate the expression of matrix metalloproteinase- 9 （ MMP- 9） and nuclear factor of-κB （NF-κB） in retinoblastoma （RB） and their relationships with differentiation and optic never infiltration for discussion their effect in invasion and metastasis of RB. METHODS： Immunohistochemical technique was adopted to examine the protein expression of MMP-9 and NF-KB in 47 cases. All the data were analyzed by statistics. RESULTS：The expression ratio of MMP-9 was 46.8%, and the expression was also significantly related to the differentiation degree（ P〈 0.05） and never infiltration （ P〈 0.05）. The positive rate of NF-κB expression was 63.8%. The expression had notable relationship with the differentiation degree （ P〈 0.05）, but not correlated with the never infiltration （P〉0.05）. The expression of NF-κB had an obvious relationship with the expression of MMP-9 （P〈0.05）. CONCLUSION： In RB, MMP-9 expressed in higher level. NF-κB was activated and had higher expression in RB. The expression of NF-KB was related to the expression of MMP-9. The activation of NF-κB can possibly promote invasion and metastasis of RB by upregulating the expression of MMP-9. Detecting the expression of MMP-9 and NF-κB may be biological indicator of invasion and metastasis of RB and can help to filter cases with high risk of metastasis.