绵羊羊膜上皮细胞治疗兔桡骨缺损模型的研究

The Study of Therapy Efficacy Applied Sheep Amnion Epithelial Cells in Rabbit Radius Defected Models

为了确定绵羊羊膜上皮细胞在体内向骨组织的分化能力,实验在分离培养绵羊羊膜上皮细胞并对其进行干细胞特性的鉴定的基础上,制作新西兰大白兔桡骨13mm骨缺损模型,随机分组对其进行注射绵羊羊膜上皮细胞实验。高剂量组:移植细胞5×107个;低剂量组:移植细胞5×106个;对照组:生理盐水。细胞移植后2、4、8周拍摄X光片观察骨缺损部位的缺损修复情况;相应时段取骨缺损部位新生骨进行组织学观察:分析骨小梁生成数量和骨的改建时期。实验结果显示,高剂量实验组在移入细胞第8周,骨缺损完全修复,且同期高剂量组新骨生成的数量和质量明显高于低剂量组,低剂量组优于对照组。由此可见,绵羊羊膜上皮细胞不仅可以在不同种动物间进行移植,而且对骨缺损有良好的修复能力。

To explore the potentials differentiation of sheep amnion epithelial cells into bone in vitro, the sheep amnion epithelial cells of stem cells properties were injected into New Zealand rabbit radius defected models and then observed the radius restoration. The sheep amnion epithelial cells were cultured and made morphological identifications. The rabbit＇ s left 13-centimetre long radius defected models were artificially divided into high dose group, low dose group and control group randomly. Sheep amnion epithelial cells with the dense of 5×10^7 and 5 × 10^6 in 5ml saline and 5ml saline were injected into high dose group of rabbits, low dose group of rabbits and control group of rabbits respectively. Took the models for X-ray in 2 weeks, 4 weeks and 8 weeks after the sheep amnion epithelial cells transplantations respectively and observed the radius restorations. At the mean time, the radius defected areas were observed by histological methods, and the trabecular bones generating quantities and the bone remodeling time were analysised. Defected radiuses in high dose group of models were restored completely at 8 weeks after the sheep amnion epithelial cells transplantations. Meanwhile, the quality and quantities of trabecular bones in high dose group were better and higher than in the low dose group and it was the same situation between the low dose group and the control group. Thus it can be seen that the sheep amnion epithelial cells can not only be transplanted among various species of animal, but also are effective for defective bone restoration.