猪蛋白酪氨酸磷酸酶-1B基因第7内含子多态性与猪脂肪沉积性状的关联性

Association of Polymorphisms of Porcine Protein Tyrosine Phosphatese 1B Gene Intron 7 with Fat Deposition Traits

本研究旨在分析蛋白酪氨酸磷酸酶-1B(PTP-1B)基因部分SNP与猪脂肪沉积性状的关联性。选取国外瘦肉型猪种大约克夏16头、长白猪21头和中国脂肪型地方猪种梅山猪22头以及"大白×梅山"F2代资源家系213头为研究材料,利用PCR-Eco88I-RFLP方法检测PTP-1B基因第7内含子序列存在G/A突变的多态性,并进行方差分析。结果表明,大白和长白猪种为单一的A等位基因,而梅山猪种以杂合子GA基因型为主,对213头"大白×梅山"F2代资源家系进行性状关联分析,该位点与猪板油质量、内脂率、胸腰椎间背膘厚等脂肪沉积性状显著相关(P<0.05),且杂合子GA基因型个体的板油质量和内酯率都最低(GA基因型个体<GG基因型个体<AA基因型个体),这可能是杂种优势的体现。而GG基因型个体的胸腰椎间背膘厚最低(GG基因型个体<GA基因型个体<AA基因型个体)。结果提示,提高G等位基因频率可显著减少脂肪沉积,该SNP可能是改良猪脂肪沉积性状的重要分子标记位点。

To discuss the association of polymorphism of porcine protein tyrosine phosphatese 1B gene with fat deposition traits,in this study,the sequence of intron 7 of the porcine PTP-1B gene was obtained from Large White,Landrace and Meishan pigs.Sequence comparison revealed a G/A mutation which was detected by PCR-Eco88 I-RFLP.The allele distribution revealed that Large White and Landrace pigs had only A allele,and GA was preponderant genotype in Meishan pigs.Association analysis between the polymorphism of PTP-1B gene and fat deposition traits was performed in 213 Large White × Meishan F2 pigs.Significant associations of the genotypes with fat deposition traits such as leaf fat weight（LFW）,internal fat rate（IFR） and thorax-waist fat thickness were found.The individuals with GA genotype had lowest leaf fat weight and internal fat rate than the individuals with the other genotypes（GA〈GG〈AA）,this might be reflection of heterosis.The individuals with GG genotype had lowest thorax-waist fat thickness than the individuals with the other genotypes（GG〈GA〈AA）.These results indicate that allele G is significantly associated with the decreasing of fat deposition,the polymorphic site can be used as the candidate site affecting pig fat deposition traits.