猪SLA-DRA基因外显子2多态性及其与仔猪腹泻的关联分析

Polymorphism of SLA-DRA Gene Exon 2 and Its Correlation with Piglet Diarrhea

为探索SLA-DRA基因作为猪抗病育种分子标记的可能性,本研究采用PCR-SSCP和克隆测序方法对大白、长白和杜洛克共216头猪的SLA-DRA基因外显子2进行了多态性研究,分析了该基因与仔猪腹泻的关联性。结果,在SLA-DRA外显子2上检出了3个等位基因和6种基因型;6种基因型(AA、AB、BB、AC、BC和CC)在大白猪和长白猪中都存在,而在杜洛克猪中只检出4种基因型(AA、BB、AB和BC)。杜洛克猪与大白猪和长白猪间基因型分布均差异极显著(P<0.01);3个品种的基因型分布均处于Hardy-Weinberg平衡状态(P>0.05)。最小二乘法分析表明,品种和性别对仔猪腹泻影响不显著(P>0.05),基因型与仔猪腹泻显著相关(P<0.05);AA和BB基因型个体腹泻评分的最小二乘均值均显著高于AC和CC基因型个体(P<0.05)。本研究表明,SLA-DRA基因不同基因型对仔猪腹泻有着重要的影响,可作为猪抗病育种应用中的一个潜在遗传标记。

In order to explore the possibility using SLA-DRA gene as genetic marker of disease resistance,the correlation between polymorphism of SLA-DRA gene exon 2 and piglets diarrhea were detected in 216 piglets from Landrace,Large White and Duroc pigs by PCR-SSCP and sequencing.Three alleles and six genotypes were detected in the SLA-DRA gene exon 2.Six genotypes（AA,AB,BB,BC,AC and CC） were both found in Landrace and Large White pigs,but only four genotypes（AA,AB,BB and BC） were detected in the Duroc pigs.The genotype distribution was significantly different among Duroc and other two pig breeds（Landrace and Large White pigs）（P〈0.01）.The genotype distributions of three breeds were all in the Hardy-Weinberg equilibrium（P〉0.05）.The least square analysis indicated that genotype significantly affected the diarrhea of piglets（P〈0.05）,but the breed and gender showed no significant influence（P〉0.05） on the diarrhea of piglets.The piglet diarrhea score of individuals with genotype AA and BB were significantly higher than that of individuals with genotype AC and CC（P〈0.05）.Therefore,it can be concluded that genotypes of SLA-DRA gene had significant influence on piglet diarrhea,and which might be a potential genetic marker in swine breeding.