犬氧化损伤诱发白内障模型及其评价标准的研究

The Research of Cataract Model Induced by Oxidative Damage and Evaluation Standards on Dogs

犬白内障是常见的眼科疾病,发病率高,严重影响犬的视力。有研究表明氧化损伤是重要的发病机制之一,目前对其疾病模型与发病机理研究较少。本研究旨在建立犬老年性白内障动物模型,为其后续机理研究奠定基础。本试验选取健康成年犬10只,随机选取6只为模型组,4只为对照组,用Fenton液造模。裂隙灯观察晶状体的混浊情况,光镜和透射电镜观察形态结构的变化,测定晶状体生化指标的改变,判定造模结果。与对照组相比,模型组晶状体混浊度变化明显,光镜和电镜下能观察到凋亡现象,T-SOD、GSH-Px和MDA值差异显著(P<0.05)。本试验证明用Fenton液囊袋内注射即可成功建立犬老年性白内障疾病模型。

Cataract,which severely affects the vision and has high incidence,is one of the common ophthalmic diseases on canine.Recent research showed that many pathogenesis gave rise to cataract formation,among which oxidative damage was an important factor.However,there was little research focused on the establishment of cataract models on canine.The aim of our study was to establish senile cataract models,which could lay a foundation for the further research.This research picked up 10 healthy dogs,randomly selected 6 dogs as model group and the rest as control group.We used Fenton solution to establish the model and judged it success or not from the following methods： checked the lens with slit lamp microscope,observed the structures of lens by light microscope and TEM,and determined the changes of biochemical indicators.Compared with the control group,the lens cloudy degree of model group changed remarkably,apoptosis phenomenon could be observed with light microscope and TEM in model group,the T-SOD,GSH-Px and MDA values in model group were significantly different（P〈0.05）.The results proved that the senile model can be successfully built through intracystic injection with fenton solution.