双室共培养中人羊膜上皮细胞诱导分化为腺泡细胞样细胞研究

Induced differentiation of human amniotic epithelial cells into acinar cells using a double chamber system

目的:研究人羊膜上皮细胞(hAECs)体外诱导分化为唾液腺腺泡细胞样细胞的可能性。方法:分离hAECs并体外培养、鉴定。与8 d龄SD大鼠下颌下腺细胞(SMGCs)在双室共培养系统中共培养。免疫细胞化学、实时荧光定量RT-PCR等方法检测α-淀粉酶及其mRNA的表达。结果:双室共培养1、2周,hAECs中α-淀粉酶mRNA的表达量分别为共培养前的3.38倍及6.6倍。结论:hAECs具有向唾液腺腺泡细胞样细胞分化的能力。

Objective： To investigate the differentiation potential of human amniotic epithelial cells（hAECs） into acinar cells in vitro.Methods： hAECs were cultured and identified in vitro.hAECs were cultured on the bottom surface of lower compartment with submandibular gland acinar cells（SMGCs） of 8-day-old SD rat on the microporous membrane in upper compartment using a double chamber system.The expression of α-amylase was determined by immunocytochemical method and fluorescent real-time PCR after 1 and 2 weeks culture respectively.Results： The expression of α-amylase in hAECs was increased by 3.38-fold and 6.6-fold after co-culture for 1 and 2 weeks respectively.Conclusion： hAECs possess the potential to transdifferentiation into acinar cells in vitro.