槲皮素壳聚糖被膜治疗口腔溃疡的实验研究

An experimental study of quercetin chitosan composite film in the treatment of oral ulcer

目的:制备槲皮素壳聚糖复合药膜(quercetin chitosan composite film,QUCS),观察其对口腔溃疡愈合过程的影响。方法:以壳聚糖(chitosan,CS)为成膜材料,加入槲皮素(Quercetin,QU)制成口腔溃疡复合膜。采用NaOH烧灼法制备大鼠口腔溃疡模型。60只大鼠随机均等分为基质组(CS,不加槲皮素的膜)、复合膜(QUCS)组、冰硼散(BPS)组和空白对照组(control)。各药均2次/d,直至溃疡完全愈合为止。对每组溃疡鼠进行大体观察,测量溃疡面积及溃疡愈合速度,肉眼观察溃疡面水肿充血程度,愈合情况。给药4 d后各组随机取5只脱颈处死,行病理组织学观察,记录溃疡区口腔黏膜损伤程度。结果:与对照组比较QUCS组平均愈合时间、平均愈合速度、溃疡面积、溃疡充血水肿程度以及溃疡区口腔黏膜损伤程度明显缩短或减轻(P<0.05,P<0.01)。在溃疡表面感染程度、溃疡平均愈合时间及充血水肿程度上QUCS组和BPS组之间无统计学差异。结论:QUCS对NaOH所致大鼠口腔溃疡具有明显的治疗作用。

Objective： To study the effect quercetin chitosan composite film（QUCS） in the treatment of oral ulcer.Methods： QUCS was prepared.Oral ulcer animal model was induced by NaOH in sixty rats.The rats were divided into 4 groups（n=15） and treated by chitosan membrance（CS group）,QUCS（QUCS group）,Bingpeng San（BPS group） and without treatment（control group） respectively.The treatment was conducted 2 times a day,until the ulcer was healed.The effects were evaluated by the ulcer area,healing speed and pathologic examination.Results： The shortest ulcer healing time,most ulcer area decrease,least edema and least pathological damage of oral membrane with ulcer were observed in QUCS group（P〈0.05,P〈0.01）.Conclusion： QUCS is effective in the treatment of oral ulcer induced by NaOH in rats.