STAT3信号通路调控Bcl-2成员在非小细胞肺癌组织中的表达

Expression of Bcl-2 Family in Non-small Cell Lung Cancer by STAT3 Signal Pathway

目的探讨非小细胞肺癌组织中STAT3、Mcl-1和Bax的表达及与细胞凋亡之间的关系。方法应用SABC免疫组化法检测43例非小细胞肺癌手术标本和15例正常肺组织中的STAT3、Mcl-1和Bax的表达。用Tunel法检测细胞凋亡,用凋亡指数(AI)评估细胞凋亡情况。结果肺癌中STAT3、Mcl-1的阳性表达率高于正常肺组织(P<0.05),肺癌组织中Bax的表达略低于正常肺组织,但两者之间无明显统计学差异(P>0.05)。肺癌中STAT3阳性表达率与分化程度相关,Bax阳性表达率与临床分期及有无淋巴结转移有关,Mcl-1的表达与肿瘤组织学类型有关(P<0.05)。肺癌组织的凋亡指数(AI)明显高于正常组织,与肿瘤分化程度有关,且STAT3表达阳性组织AI值明显低于STAT3阴性组(P<0.05)。结论 STAT3蛋白通过调控Mcl-1和Bax发挥其在非小细胞肺癌组织中的抗凋亡作用。

Objective To explore the expression of STAT3,Mcl - 1 and Bax in non - small cell lung cancers（NSCLC） , and study their relationship with cell apotosis. Methods The immunohistochemical stain SABC method was applied to investigate thee expression of STAT3 ,Mcl - 1 and Bax in samples of 43 cases NSCLC and 15 normal lung tissues. Tunel method was applied to detect the apoptosis. The apoptosis index （AI） was defined. Results The expression of STAT3 and Mcl - 1 was significantly higher in lung cancer tissues than normal tissues （P 〈 0.05 ） ,while bax was slightly lower in lung cancer tissues than normal tissues, but there was no significant difference between them （ P 〉 0.05 ）. The expression of STAT3 was related with differentiation grade of cancers, and the positive rate of Bax had corre- lation with clinical stage and lymphnode metastasis. The expression of Mcl- 1 correlated with histological differentiation（P 〈 0.05 ）. The AI of cancer tissues was higher than that of normal tissues,and related to differentiation degree of cancers. The AI values in lung cancer samples with possitive response to STAT3 were lower than negative ones （P 〈 0.05）. Conclusion STAT3 protein was in the processing of antiapoptosis in NSCLC by Mcl- 1 and Bax.