Th17细胞及其相关细胞因子在幽门螺杆菌感染中的作用研究

Role of Thl7 cells and related cytokines in Helicobacter pylori infection.

目的探讨Th17细胞及其相关细胞因子在幽门螺杆菌（H.pylori）感染中的作用。方法建立幽门螺杆菌感染的小鼠模型，同时设立治疗组与对照组。HE染色评价小鼠胃组织学改变；RT-PCR法检测胃组织IL-17mRNA、IL-23mRNA表达水平；ELISA法检测胃组织匀浆上清IL-17、IL-23含量；FCM检测脾脏单细胞悬液中Th17细胞应答情况。结果与对照组相比，Htrylori感染组小鼠胃组织IL-17、IL-23在mRNA及蛋白水平表达均升高，脾淋巴细胞中Thl7细胞比例显著升高；而且H.pylori感染组小鼠IL-17、IL-23mRNA和蛋白含量以及脾淋巴细胞中Th17细胞比例随感染时间延长而增加；治疗组小鼠IL-17、IL-23表达量及脾淋巴细胞中Thl7细胞比率，与治疗前即感染后4周的小鼠相比较均有所下降；感染后不同时期小鼠胃黏膜的炎症程度与IL-17、IL-23的表达量存在正相关。结论H.pylori感染后可以诱导Thl7细胞应答且IL-17、IL-23表达均上调；H.pylori感染后胃炎程度与胃组织IL-17、IL-23含量存在正相关。

Objective To investigate the role of Thl7 cells and related cytokines in Helicobacter pylori（H, pylori） infection. Methods To establish a mouse model of H. pylori infectious gastritis. Meanwhile, a treatment group and control group were set up. The histological changes in the gastric mucosa were observed and scored under light microscopy; The expression of IL-17 and IL-23 mRNAs as well as protein in gastric tissue was detected by RT-PCR and ELISA, respectively; Thl7 cells in mice splenocyte were evaluated using the flow cytometry （FCM） method. Results We found significantly higher levels of IL-17 and IL- 23 protein and mRNA levels in supernatant of gastric tissue homogenates of infection group as compared to controls and Thl7 cells in spleen from mice of H, pylori infection group were all obviously higher than that of control group; IL-17 mRNA, IL-23 mRNA and the ratio of Thl7 cells in mice splenocyte of H. pylori infection group mice increased gradually with the time prolonged; The levels of both mRNA and protein levels of IL-17 and IL-23 decreased significantly in the treatment group compared with pre-treatment; There was a positive correlation between IL-17 and IL-23 expression levels of mice gastric mucosa and gastric inflammation degree. Conclusion H, pylori infection induced the immune response of Thl7 cells ; The levels of IL-17 and IL-23 increased in gastric mucosa of mice after H. pylori infection ; The degree of H, pylori -infected mice gastric inflammation was positive correlation with the levels of IL-17 and IL-23 of gastric mucosa.