短时重复游泳调节SAM8鼠AMPA受体GluR1亚单位的磷酸化

A transient,but repeated swimming regulating the GluR1 phosphorylation of AMPA receptor in SAM8 mice

目的观察短时重复游泳训练对SAM鼠AMPA受体GluR1亚单位磷酸化的影响,探讨运动改善脑功能的可能机制。方法选取3月龄SAMP8(prone/8)亚系为研究对象,运动模型采用2 w游泳方案:2次/d,每次6 min的游泳,结束后给予浴巾擦干放回鼠笼;对照组则在相同时间每天给予两次相同的浴巾安抚刺激。采用Western印迹方法,检测SAM8鼠海马和皮层AMPA受体GluR1亚单位Ser831和Ser 845位点的磷酸化水平的变化。结果 SAMP8海马、皮层中AMPA受体GluR1亚单位Ser831和Ser845磷酸化水平与对照组相比均增加(P<0.05)。结论 2 w的短时间重复游泳运动作为一种应激诱导剂促进了AMPA受体的活化,这可能是运动改善脑功能的机制之一。

Objective To investigate the effects of a transient, but repeated swimming on the GluR1 phosphorylation of AMPA receptor in SAM8 mice, and explore the possible molecular mechanisms for exercise improving brain function. Methods 16 male SAM8 mice were equally randomized into normal and swimming groups. Swimming protocol was conducted twice a day for 6 min, each for a total of 14 days. After swimming, the mice were ＆led with a towel and placed back into their original cage. A control group of animals was handled for 6 s, wrapped in a towel twice a day tbr 14 days to simulate the handle after swimming. The phosphorylation of GluR1 at Ser831 and Ser845 were measured by Western blot. Results Compared with normal group, both in cortex and in hippocampus, the phosphorylation degree of GluR1 at Ser831 and Ser845 were significantly increased （ all P 〈 0. 01 ）. Conclusions 2-week swimming protocol may be a stress inducer which lead to the activation of AMPA receptor, and that may be the one of mechanisms of exercise benefiting brain function.