针药联合抑制缺血再灌注大鼠PI3K/A_(kt)信号通路的相关性研究

The study on Inhibitting Ischemia - reperfusion Rats' PI 3K /A_(kt) Signal Pathways by Acupuncture Combining Medicine

目的:通过检测缺血再灌注大鼠心肌细胞凋亡情况,以及磷酸化Akt(p-Akt)等指标,探讨中药预处理联合针刺后对缺血再灌注心肌细胞的大鼠模型影响及作用机制。方法:健康清洁级wistar大鼠24只,随机分为6组:缺血再灌注组、心脑通络液预处理组、针刺后处理组、心脑通络液预处理组+针刺后处理组。建立缺血再灌注损伤模型。免疫组化检测p-Akt、RT-PCR检测AktmRNA水平、West-ern blot检测Akt、p-Akt的变化。结果:应用PI3K/Akt信号通路阻断剂渥曼青霉素后,心脑通络液加针刺降低心肌细胞凋亡指数的作用被抑制,p-Akt、p-Akt/Akt表达减少(P>0.01),而AktmRNA含量未见明显变化。结论:1)心脑通络液预处理联合针刺后处理没有改变缺血再灌注大鼠心肌中AktmRNA含量。2)心脑通络液预处理联合针刺后处理使大鼠心肌中p-Akt、p-Akt/Akt表达增多,应用PI3K/Akt信号通路阻断剂渥曼青霉素后,心脑通络液预处理联合针刺后处理降低心肌细胞凋亡的作用被抑制,p-Akt、p-Akt/Akt表达减少,说明心脑通络液预处理联合针刺后处理的心肌保护作用是通过PI3K/Akt通路介导实现的。

Objective： To explore the curative effect and mechanism of Xinnao Tongluo liquid preconditioning and elec- troacupuncture （EA） postconditioning with ischemic reperfusion of rats heart through apoptotic index, p - Akt. Methods ： All rats were randomly divided in four groups： IR group, Xinnao Tongluo liquid preconditioning group, EA postcondi- tioning group, Xinnao Tongluo liquid preconditioning and EA postconditioning group. Establishment of ischemia reperfu- sion injury model. Test the variety of apoptotic index p - Akt by immunol histochemistry , RT - PCR and western blot. Results： Xinnao Tongluo liquid preconditioning, EA postconditioning can decrease the infarct size and apoptotic index, increase p - Akt, Xinnao Tongluo liquid preconditioning and EA postconditioning group was better than other groups. Conclusion ： 1. Xinnao Tongluo liquid preconditioning and EA postconditioning affect the contents of AktmRNA . 2. Xin- nao Tongluo liquid preconditioning and EA postconditioning can increase the expression of p - Akt, and it shows that PI3K/Akt pass way participate in the role of lightening ischemic reperfusion injury .