刺五加水煎液合并DA受体拮抗剂改善小鼠睡眠作用的实验研究

Acanthopanax Senticosus Combined with Dopamine Receptor Antagonist for Improving Mice Sleep

目的:探讨刺五加水煎液改善小鼠睡眠作用的机制。方法:采用酶联免疫吸附法,观察刺五加水煎液(32g.kg-1)对小鼠脑内多巴胺(DA)含量的影响。应用戊巴比妥钠致小鼠睡眠的实验方法,观察小鼠给予阈下剂量刺五加水煎液合并多巴胺D1受体拮抗剂SCH23390,以及刺五加水煎液合并多巴胺D2受体拮抗剂氟哌啶醇(Haloperidol)后小鼠睡眠潜伏期和睡眠时间。结果:刺五加水煎液对小鼠全脑DA的含量与空白组相比无显著性差异(P>0.05)。与空白组比较,给予阈下剂量刺五加水煎液(8g.kg-1)合并阈下剂量SCH23390(7μg.kg-1)可显著性延长小鼠的睡眠时间(P<0.01)和缩短睡眠潜伏期(P<0.01);给予阈下剂量刺五加水煎液(8g.kg-1)合并阈下剂量Haloperidol(0.7mg.kg-1)也可显著性延长小鼠的睡眠时间(P<0.01)和缩短入睡潜伏期(P<0.01)。结论:刺五加水煎液改善睡眠的作用可能是通过与多巴胺D1和D2受体拮抗剂的协同作用而产生的。

Objective： To study the machanism of the effect of Acanthopanax senticosus on improving mice sleep. Meth- ods ： The content of DA in the whole brain of mice administrated by Acanthopanax senticosus （ 32g. kg - t ） was estimated with ELISA. This research observed the latency and duration of sleep of mice which was narcosised by pentobarbital after being administrated with subthreshold dose of Acanthopanax senticosus combined with dopamine D1 receptor antagonist SCH23390 or dopamine D2 receptor antagonist Haloperidol. Results： The content of DA in the whole brain of mice ad- ministrated by Acanthopanax senticosus showed no significant difference compared with the control group （ P 〉 0.05 ）. Contrasting with the blank group, the results showed that SCH23390（7 μg· kg-1） significantly prolonged the time of fall- ing asleep（P 〈0.01 ）and shortened the duration sleep latency（P 〈0.01 ）in mice which were administrated with sub- threshold dose of Acanthopanax senticosus （8g.kg-1） ; Haloperidol （7mg.kg-1） was able to significantly prolong the time of falling asleep （P 〈 0.01 ）and shorten the duration sleep latency （P 〈 0.01 ）in mice which were administrated with subthreshold dose of Acanthopanax senticosus （8g. kg-1）. Conclusion： The synergic effect of Acanthopanax senticosus combined with dopamine DA1 ,DA2 receptor antagonist seems to be one of the reasons why Acanthopanax senticosus can extend sleep time.