高效液相色谱波长切换法同时测定桑叶中多指标成分的含量

Simultaneously Determination of Wavelength Switching of Multi-target Ingredients in Mulberry Leaves(Morus alba.L)by HPLC

目的:建立桑叶药材中槲皮素、山奈酚、槲皮苷、芦丁、异槲皮苷、绿原酸、东莨菪内酯的含量测定方法。方法:采用HPLC外标法。采用色谱柱:AgilentC18柱(150mm×4.6mm,5μm),以乙腈为流动相A,以0.1%磷酸溶液为流动相B,梯度洗脱。检测波长分别切换为350nm,298nm,350nm,255nm,360nm,310nm。结果:槲皮素、山奈酚、槲皮苷、芦丁、异槲皮苷、绿原酸、东莨菪内酯的线性范围分别为0.0958～0.6065、0.0318～0.2016、0.1157～0.7326、0.1110～0.7032、0.1883～1.1923、0.2945～1.8652、0.1892～1.1979,平均回收率分别为102.8%、101.8%、99.5%、103.8%、99.1%、100.5%、98.7%。结论:本方法简便、准确,在同一色谱条件下可同时测定桑叶中槲皮素、山奈酚、槲皮苷、芦丁、异槲皮苷、绿原酸、东莨菪内酯的含量。

Objective： To determine the contents of quercetin, kaempferol, quercitrin, rutin, isoquercetin, chlorogenic acid and scopoletin in mulberry leaves by HPLC. Methods：Anextemal standard method was adopped by HPLC with Agilent C18 column（ 150mm×4. 6mm,5 μm）as fixed phase,The mobile phase A was acetonitrile and the mobile phase B was 0. 1% phosphoric acid. The detection wavelength respectively was at 350nm,298nm,350nm,255nm,360nm,310nm. Results： Quer- cetin had a good linearity in the range of 0. 0958-0. 6065mg/ml. The average recovery rate was 102. 8%. Kaempferol had a good linearity in the range of 0. 0318-0. 2016 mg/ml. The average recovery rate was 101.8%. Quercitrin had a good lincarity in the range of 0. 1157-0. 7326 mg/ml. The average recovery rate was 99.5%. Rutin had a good lineafity in the range of 0. 1110-0. 7032 mg/ml. The average recovery rate was 103. 8%. Isoquercetin had a good linearity in the range of 0. 1883-1. 1923 mg/ml. The average recovery rate was 99. 1%. Chlorogenic acid had a good linearity in the range of 0. 2945-1. 8652 mg/ml. The average recovery rate was 100. 5%. Seopoletin had a good linearity in the range of 0. 1892-1. 1979 mg/ml. The average recovery rate was 98.7%. Concluslon：The method is simple and accurate. It can be used to determine quercetin , kaempferol ,quercitrin,rutin,isoquercetin,chlorogenic acid and scopolctin together with the same chromatogram cond/tion.