猪精液中五种病毒的初步检测

Detection of Five Viruses in Boar Semen

为探明猪精液在疫病传播中的作用,应用PCR和RT-PCR技术分别从发病猪场和未发病猪场采集种公猪精液进行猪瘟病毒(CSFV)、猪繁殖与呼吸综合征病毒(PRRSV)、猪伪狂犬病病毒(PRV)、猪圆环病毒2型(PCV-2)和牛病毒性腹泻病毒(BVDV)的检测。结果发现,发病猪场的种公猪精液中CSFV、PRRSV、PRV、PCV-2的感染率分别为30.0%、50.0%、4.0%和30.0%,而未发病猪场的感染率分别为4.0%、20.0%、0.0%和8.0%,这表明精液是多种猪病原传播的良好媒介,提示种公猪对于疫病的发生和传播起着重要作用。

To clarify the pathogenicity of boar semen , Classical swine fever virus （CSFV） , Porcine repro- ductive and respiratory syndrome virus （PRRSV） , Pseudorabies virus （PRV） , Porcine circovirus type 2 （PCV-2） and Bovine viral diarrhea virus（BVDV） were detected from boar semens collected from clinical and healthy pig farms. The detections showed that infection rate of CSFV, PRRSV, PRV,PCV-2 existed in clinical pig farms with 30.0~,50.0%,4.0~ and 30.0%, while a lower infection accounted for 4.0%, 20.0% ,0.0% and 8.0% in healthy pig farms, respectively. It indicated strongly that the boar played a role in the occurrence and spread of diseases.